诺维科夫腹水瘤细胞、小鼠淋巴瘤细胞和母鸡输卵管中N-乙酰葡糖胺基转移酶III、IV和V的活性。利用高效液相色谱法进行灵敏且特异的测定方法的应用。
N-acetylglucosaminyltransferase III, IV and V activities in Novikoff ascites tumour cells, mouse lymphoma cells and hen oviduct. Application of a sensitive and specific assay by use of high-performance liquid chromatography.
作者信息
Koenderman A H, Koppen P L, Koeleman C A, van den Eijnden D H
机构信息
Department of Medical Chemistry, Vrije Universiteit, Amsterdam, The Netherlands.
出版信息
Eur J Biochem. 1989 May 15;181(3):651-5. doi: 10.1111/j.1432-1033.1989.tb14772.x.
A specific and fast method for the determination of N-acetylglucosaminyltransferase III, IV and V activity in one assay is described. The method is based on the separation by HPLC of the three transferase products formed from the common acceptor oligosaccharide substrate GlcNAc beta 1----2Man alpha 1----3(GlcNAc beta 1----2Man alpha 1---- 6)Man beta 1----4GlcNAc. Assays are not interfered with by substances that result from enzymatic or chemical breakdown of the donor substrate UDP-[14C]GlcNAc. Using this assay system N-acetylglucosaminyltransferase III, IV and V activities were estimated in Novikoff ascites tumour cells, mouse lymphoma BW 5147 cells and hen oviduct.
本文描述了一种在一次测定中同时测定N-乙酰葡糖胺基转移酶III、IV和V活性的特异性快速方法。该方法基于通过高效液相色谱法分离由共同受体寡糖底物GlcNAcβ1----2Manα1----3(GlcNAcβ1----2Manα1---- 6)Manβ1----4GlcNAc形成的三种转移酶产物。供体底物UDP-[14C]GlcNAc的酶促或化学分解产生的物质不会干扰测定。使用该测定系统，对诺维科夫腹水肿瘤细胞、小鼠淋巴瘤BW 5147细胞和母鸡输卵管中的N-乙酰葡糖胺基转移酶III、IV和V活性进行了评估。
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