Perng Wei, Gillman Matthew W, Fleisch Abby F, Michalek Ryan D, Watkins Steven M, Isganaitis Elvira, Patti Mary-Elizabeth, Oken Emily
Obesity Prevention Program, Department of Population Medicine, Harvard Medical School and Harvard Pilgrim Health Care Institute, Boston, Massachusetts, USA.
Obesity (Silver Spring). 2014 Dec;22(12):2570-8. doi: 10.1002/oby.20901. Epub 2014 Sep 24.
To identify metabolite patterns associated with childhood obesity, to examine relations of these patterns with measures of adiposity and cardiometabolic risk, and to evaluate associations with maternal peripartum characteristics.
Untargeted metabolomic profiling was used to quantify metabolites in plasma of 262 children (6-10 years). Principal components analysis was used to consolidate 345 metabolites into 18 factors and identified two that differed between obese (BMI ≥ 95‰; n = 84) and lean children (BMI < 85‰; n = 150). The relations of these factors with adiposity (fat mass, BMI, skinfold thicknesses) and cardiometabolic biomarkers (HOMA-IR, triglycerides, leptin, adiponectin, hsCRP, IL-6) using multivariable linear regression was then investigated. Finally, the associations of maternal prepregnancy obesity, gestational weight gain, and gestational glucose tolerance with the offspring metabolite patterns was examined.
A branched-chain amino acid (BCAA)-related pattern and an androgen hormone pattern were higher in obese vs. lean children. Both patterns were associated with adiposity and worse cardiometabolic profiles. For example, each increment in the BCAA and androgen pattern scores corresponded with 6% (95% CI: 1, 13%) higher HOMA-IR. Children of obese mothers had 0.61 (0.13, 1.08) higher BCAA score than their counterparts.
BCAA and androgen metabolites were associated with adiposity and cardiometabolic risk during mid-childhood. Maternal obesity may contribute to altered offspring BCAA metabolism.
识别与儿童肥胖相关的代谢物模式，研究这些模式与肥胖及心脏代谢风险指标之间的关系，并评估其与母亲围产期特征的关联。
采用非靶向代谢组学分析对262名6至10岁儿童的血浆代谢物进行定量分析。主成分分析用于将345种代谢物整合为18个因子，并识别出肥胖儿童（BMI≥95‰；n = 84）和瘦儿童（BMI < 85‰；n = 150）之间存在差异的两个因子。随后，使用多变量线性回归研究这些因子与肥胖指标（脂肪量、BMI、皮褶厚度）和心脏代谢生物标志物（HOMA-IR、甘油三酯、瘦素、脂联素、hsCRP、IL-6）之间的关系。最后，研究母亲孕前肥胖、孕期体重增加和孕期糖耐量与后代代谢物模式的关联。
肥胖儿童中与支链氨基酸（BCAA）相关的模式和雄激素激素模式高于瘦儿童。这两种模式均与肥胖及较差的心脏代谢状况相关。例如，BCAA和雄激素模式评分每增加一个单位，HOMA-IR就相应升高6%（95%CI：1，13%）。肥胖母亲的孩子的BCAA评分比正常母亲的孩子高0.61（0.13，1.08）。
BCAA和雄激素代谢物与儿童中期的肥胖及心脏代谢风险相关。母亲肥胖可能导致后代BCAA代谢改变。
