Hosoda M, Makino S, Kawabe T, Maeda Y, Satoh S, Takami M, Mayumi M, Arai K, Saitoh H, Yodoi J
Institute for Immunology, Faculty of Medicine, Kyoto University, Japan.
J Immunol. 1989 Jul 1;143(1):147-52.
Two types of activation Ag, low affinity FcR for IgE (Fc epsilon R2)/CD23 and IL-2R (Tac/p55), were expressed and differently regulated on human eosinophilic leukemia cell lines (EoL-1 and EoL-3). Because the binding of IgE on EoL-3 cells was completely inhibited by H107 (anti-Fc epsilon R2/CD23 mAb) but not by irrelevant mAb, essentially all the low affinity Fc epsilon R2 on EoL-3 seemed to be the Fc epsilon R2/CD23 molecules. Both IL-4 and IFN-gamma enhanced the surface expression of Fc epsilon R2, whereas IL-1, IL-2, and IL-5 showed no effects, as determined by surface staining with anti-Fc epsilon R2 antibody (H107). In contrast to Fc epsilon R2 up-regulation, IL-4 and IFN-gamma showed a differential effect on the regulation of IL-2R (Tac/p55). Whereas IFN-gamma up-regulated the receptor expression of IL-2R/Tac, IL-4 did not. The result suggests that these lymphokines are involved in the different aspects of the activation pathway of the eosinophils. The possible role of Fc epsilon R2 and IL-2R on the function of eosinophils in allergic reaction is discussed.
两种激活抗原,即低亲和力IgE Fc受体(FcεR2)/CD23和IL-2受体(Tac/p55),在人嗜酸性粒细胞白血病细胞系(EoL-1和EoL-3)上表达且调控方式不同。由于EoL-3细胞上IgE的结合被H107(抗FcεR2/CD23单克隆抗体)完全抑制,而无关单克隆抗体则无此作用,因此EoL-3上基本上所有的低亲和力FcεR2似乎都是FcεR2/CD23分子。用抗FcεR2抗体(H107)进行表面染色测定,结果显示IL-4和IFN-γ均增强了FcεR2的表面表达,而IL-1、IL-2和IL-5则无此作用。与FcεR2上调相反,IL-4和IFN-γ对IL-2受体(Tac/p55)的调控表现出差异效应。IFN-γ上调了IL-2R/Tac的受体表达,而IL-4则无此作用。该结果表明这些淋巴因子参与了嗜酸性粒细胞激活途径的不同方面。本文还讨论了FcεR2和IL-2R在过敏反应中嗜酸性粒细胞功能方面可能发挥的作用。