Kaneko K, Takagi N
Department of Veterinary Obstetrics and Gynecology, Azabu University, Chuo-ku, Sagamihara, Kanagawa, Japan.
Reprod Domest Anim. 2014 Dec;49(6):985-8. doi: 10.1111/rda.12418. Epub 2014 Sep 24.
To develop an ultrasonographic assay for determining plasma progesterone concentration (P4 ) as < 1 ng/ml or ≥ 1 ng/ml, the corpus luteum (CL) area and P4 were measured in 1094 multiparous Holstein cows. The area-measuring function and frozen images were used to outline and measure CL imaged via ultrasonography, and CL area was estimated as a polygon of a continuation straight line. A significant correlation was found between CL area and P4 (p < 0.001), and this analysis resulted in the following correlation equation: y = -0.35 + 1.02x (r = 0.81). According to the correlation equation, a CL area of 1.3 cm(2) indicated a P4 of 1 ng/ml. Based on this relationship, each animal was categorized into one of six groups, groups differed based on CL area, and the area ranges were as follows: < 1.3 cm(2) (Group A), 1.3-2.2 cm(2) (Group B), 2.3-3.2 cm(2) (Group C), 3.3-4.2 cm(2) (Group D), 4.3-5.2 cm(2) (Group E) and > 5.2 cm(2) (Group F). For each group, the proportion of cows whose P4 was 1 ng/ml or more was 1.5% in Group A, 83.3% in Group B, 76.6% in Group C, 96.6% in Group D, 99.2% in Group E and 100% in Group F. There was a significant difference between Group A and the other five groups, and between Groups B or C and Groups D, E or F (p < 0.005). These results indicate that a functional CL does not exist when the CL area is less than 1.3 cm(2) and that it exists when the CL area is 3.3 cm(2) or more.
为开发一种用于测定血浆孕酮浓度（P4）低于1 ng/ml或≥1 ng/ml的超声检测方法，对1094头经产荷斯坦奶牛的黄体（CL）面积和P4进行了测量。利用面积测量功能和冷冻图像勾勒并测量经超声成像的CL，CL面积估计为连续直线构成的多边形。发现CL面积与P4之间存在显著相关性（p < 0.001），该分析得出以下相关方程：y = -0.35 + 1.02x（r = 0.81）。根据相关方程，CL面积为1.3 cm²表明P4为1 ng/ml。基于这种关系，将每头动物分为六组之一，各组根据CL面积不同，面积范围如下：< 1.3 cm²（A组），1.3 - 2.2 cm²（B组），2.3 - 3.2 cm²（C组），3.3 - 4.2 cm²（D组），4.3 - 5.2 cm²（E组）和> 5.2 cm²（F组）。对于每组，P4为1 ng/ml或更高的奶牛比例在A组中为1.5%，B组中为83.3%，C组中为76.6%，D组中为96.6%，E组中为99.2%，F组中为100%。A组与其他五组之间以及B组或C组与D组、E组或F组之间存在显著差异（p < 0.005）。这些结果表明，当CL面积小于1.3 cm²时不存在功能性CL，而当CL面积为3.3 cm²或更大时存在功能性CL。
