小鼠模型伤口愈合过程中基质成分和淋巴细胞的免疫组织化学特征

Immunohistochemical profile of stromal constituents and lymphoid cells over the course of wound healing in murine model.

作者信息

Almeida Bernadeth Moda de, Nascimento Marismar Fernandes do, Pereira-Filho Rose Nely, Melo Genecy Calado de, Santos José Cleveilton dos, Oliveira Clauberto Rodrigues de, Gomes Margarete Zanardo, Lima Sônia Oliveira, Albuquerque-Júnior Ricardo Luiz Cavalcanti de

机构信息

Tiradentes University, Aracaju, SE, Brazil.

UNIT, Aracaju, SE, Brazil.

出版信息

Acta Cir Bras. 2014 Sep;29(9):596-602. doi: 10.1590/s0102-8650201400150007.

DOI:10.1590/s0102-8650201400150007

PMID:25252206

Abstract

PURPOSE

To assess the evolution profile of the immunohistochemical expression of stromal constituents over the time-course of wound healing in a murine model.

METHODS

Surgical wounds were performed in the back of 24 Wistar rats. After three, seven, 14 and 21 days, six rats were euthanized and the wounded histologically processed to assess the immunohistochemical expression of CD3, CD20, CD31, α-SMA and type-I collagen. Non-injured skin samples (NSS) were used as control. Data were subjected to statistical analysis using ANOVA and Tukey test.

RESULTS

The mean of CD3 and CD20 positive cells in the wounds was significantly higher than in NSS at seven and 14 days (p<0.001). The blood vessels content was significantly lower than in NSS (p<0.05) at three days, but increased at seven and 14 days (p<0.01). The mean of α-SMA positive cells at seven, 14 and 21 days was higher than in NSS (p<0.05). The relative content of type I collagen increased from three to 21 days, but remained lower than in NSS (p<0.05).

CONCLUSIONS

Lymphoid cells, myofibroblasts and microvessels contents varied over the time-course of wound healing, with peak at seven days and progressive reduction until 21 days. The type I collagen content increased over time.

摘要

目的

评估小鼠模型伤口愈合过程中基质成分免疫组化表达的演变情况。

方法

对24只Wistar大鼠背部进行手术造创。在术后3天、7天、14天和21天，处死6只大鼠，对伤口进行组织学处理，以评估CD3、CD20、CD31、α -平滑肌肌动蛋白（α - SMA）和I型胶原的免疫组化表达。未受伤的皮肤样本（NSS）用作对照。数据采用方差分析和Tukey检验进行统计分析。

结果

伤口中CD3和CD20阳性细胞的平均值在第7天和14天时显著高于NSS（p<0.001）。血管含量在第3天时显著低于NSS（p<0.05），但在第7天和14天时增加（p<0.01）。第7天、14天和21天时α - SMA阳性细胞的平均值高于NSS（p<0.05）。I型胶原的相对含量从第3天到21天增加，但仍低于NSS（p<0.05）。