Sharif Mahjabeen, Khan Bushra Tayyaba, Ajmal Khalida, Anwar Mohammad Asim
J Pak Med Assoc. 2014 Aug;64(8):932-5.
To study the magnitude of insulin-mediated airway hyper-reactivity and to explore the protective effects of salbutamol in inhibiting the insulin-induced airway hyper-responsiveness on tracheal smooth muscle of guinea pigs in vitro.
The quasi-experimental study was conducted at the Pharmacology Department of Army Medical College, Rawalpindi, in collaboration with the Centre for Research in Experimental and Applied Medicine from December 2011 to July 2012. It used 18 healthy Dunkin Hartely guinea pigs of either gender. Effects of increasing concentrations of histamine (10-8-10-3M), insulin (10-8-10-3 M) and insulin pre-treated with salbutamol (10-6 M) were observed on isolated tracheal strip of guinea pig in vitro by constructing cumulative concentration response curves. The tracheal smooth muscle contractions were recorded with Transducer on Four Channel Oscillograph. Mean and standard error of mean were calculated. SPSS 16 was used for statistical analysis.
Histamine and insulin produced a concentration-dependent reversible contraction of isolated tracheal muscle of guinea pig. The mean of maximum amplitudes of contraction with histamine, insulin and insulin pretreated with salbutamol were 92.5 +/- 1.20 mm, 35 +/- 1.13 mm and 14.55 +/- 0.62 mm respectively. Salbutamol shifted the concentration response curve of insulin to the right and downwards.
Salbutamol significantly reduced the insulin mediated airway hyper-reactivity in guinea pigs, suggesting that pre-treatment of inhaled insulin with salbutamol may have clinical implication in the amelioration of its potential respiratory adverse effects such as bronchoconstriction.
研究胰岛素介导的气道高反应性的程度，并探讨沙丁胺醇在体外对豚鼠气管平滑肌抑制胰岛素诱导的气道高反应性的保护作用。
2011年12月至2012年7月，在拉瓦尔品第陆军医学院药理学系与实验与应用医学研究中心合作进行了这项准实验研究。使用了18只健康的 Dunkin Hartely 豚鼠，雌雄不限。通过构建累积浓度反应曲线，观察不同浓度组胺（10-8 - 10-3M）、胰岛素（10-8 - 10-3M）以及用沙丁胺醇（10-6M）预处理的胰岛素对体外分离的豚鼠气管条的影响。用换能器在四通道示波器上记录气管平滑肌收缩情况。计算平均值和平均标准误差。使用SPSS 16进行统计分析。
组胺和胰岛素使豚鼠离体气管肌肉产生浓度依赖性可逆收缩。组胺、胰岛素和用沙丁胺醇预处理的胰岛素的最大收缩幅度平均值分别为92.5±1.20mm、35±1.13mm和14.55±0.62mm。沙丁胺醇使胰岛素的浓度反应曲线向右下方移动。
沙丁胺醇显著降低了豚鼠胰岛素介导的气道高反应性，提示用沙丁胺醇预处理吸入胰岛素可能对改善其潜在的呼吸不良反应如支气管收缩具有临床意义。
