Sharif Mahjabeen, Tayyaba Khan Bushra, Bakhtiar Salman, Anwar Mohammad Asim
Department of Pharmacology and Therapeutics, National University of Sciences and Technology (NUST) Islamabad, Army Medical College Rawalpindi, Pakistan.
Department of Pharmacology and Therapeutics, Army Medical College Rawalpindi, National University of Sciences and Technology (NUST) Islamabad, Pakistan.
Iran J Pharm Res. 2015 Spring;14(2):567-71.
Inhalational insulin was withdrawn from the market due to its potential to produce airway hyper-reactivity and bronchoconstriction. So the present study was designed to explore the acute effects of insulin on airway reactivity of guinea pigs and protective effects of salbutamol and beclomethasone against insulin induced airway hyper-responsiveness on isolated tracheal smooth muscle of guinea pig. Effects of varying concentrations of insulin (10(-7) to 10(-3) M), insulin pretreated with fixed concentration of salbutamol (10(-7) M) and beclomethasone (10(-6) M) were studied on isolated tracheal tissue of guinea pig by constructing cumulative concentration response curves. Changes in tracheal smooth muscle contractions were recorded on four channel oscillograph. The mean ± SEM of maximum amplitudes of contraction with increasing concentrations of insulin, insulin pretreated with fixed concentration of salbutamol and beclomethasone were 35 ± 1.13 mm, 14.55 ± 0.62 mm and 22 ± 1.154 mm respectively. Although salbutamol and beclomethasone both had a profound inhibitory effect on insulin induced airway hyper-reactivity, yet salbutamol is more efficacious than beclomethasone. So we suggest that pretreatment of inhaled insulin with salbutamol may be preferred over beclomethasone in amelioration of its potential respiratory adverse effects such as bronchoconstriction.
吸入性胰岛素因有可能导致气道高反应性和支气管收缩而退出市场。因此，本研究旨在探讨胰岛素对豚鼠气道反应性的急性影响，以及沙丁胺醇和倍氯米松对胰岛素诱导的豚鼠离体气管平滑肌气道高反应性的保护作用。通过构建累积浓度反应曲线，研究了不同浓度胰岛素（10⁻⁷至10⁻³M）、用固定浓度沙丁胺醇（10⁻⁷M）和倍氯米松（10⁻⁶M）预处理的胰岛素对豚鼠离体气管组织的影响。在四通道示波器上记录气管平滑肌收缩的变化。随着胰岛素浓度增加、用固定浓度沙丁胺醇和倍氯米松预处理的胰岛素浓度增加，收缩最大幅度的平均值±标准误分别为35±1.13mm、14.55±0.62mm和22±1.154mm。虽然沙丁胺醇和倍氯米松对胰岛素诱导的气道高反应性均有显著抑制作用，但沙丁胺醇比倍氯米松更有效。因此，我们建议在改善吸入性胰岛素潜在的呼吸不良反应如支气管收缩方面，用沙丁胺醇预处理可能比用倍氯米松更可取。
