Nguyen Minh Hong, Ojima Yoshihiro, Taya Masahito
Division of Chemical Engineering, Graduate School of Engineering Science, Osaka University.
Biocontrol Sci. 2014;19(3):147-50. doi: 10.4265/bio.19.147.
Scanning electron microscopy revealed that the rpoS-deficient cells of E. coli K-12 BW25113 (ΔrpoS) increased the number of flagella on the cell surfaces. However, the quantitative analysis of cell colonization showed that the increased number of flagella on ΔrpoS cell surfaces did not cause the enhancement of cell colonization on the surfaces of polyvinyl chloride (PVC), polypropylene (PP) and polystyrene (PS) after 24 h of incubation at 37℃. To facilitate the enhanced expression of curli, the csgA gene was introduced into the ΔrpoS cells. The transformed cells rich in flagella and curli on the cell surfaces were found to make colonies 2-3 times larger than both the wild type and ΔrpoS cells on the PVC, PP and PS surfaces at 37℃. It was thus verified that the reinforcement of csgA gene in the ΔrpoS cells induced the enhanced colonization on the solid surfaces with the increased flagellum and curli expressions.
扫描电子显微镜显示，大肠杆菌K-12 BW25113的rpoS缺陷型细胞（ΔrpoS）增加了细胞表面鞭毛的数量。然而，细胞定殖的定量分析表明，在37℃孵育24小时后，ΔrpoS细胞表面增加的鞭毛数量并未导致其在聚氯乙烯（PVC）、聚丙烯（PP）和聚苯乙烯（PS）表面的细胞定殖增强。为促进卷曲菌毛的增强表达，将csgA基因导入ΔrpoS细胞。发现在37℃下，细胞表面富含鞭毛和卷曲菌毛的转化细胞在PVC、PP和PS表面形成的菌落比野生型和ΔrpoS细胞大2至3倍。因此证实，在ΔrpoS细胞中增强csgA基因可通过增加鞭毛和卷曲菌毛的表达诱导在固体表面的定殖增强。
