Petrushina L I, Kolosnitsyna N V
Vopr Pitan. 1989 Mar-Apr(2):51-4.
Eight nutrient media used for St. aureus phage-typing were assessed in parallel experiments. 22 test strains of St. aureus with previously known bacteriophage formulas and 44 strains isolated from different sources during food poisonings were subjected to typing. Bacteriophages were employed in the test dose (TD) and in 10 TD. Marked relationship was established between the degree of lytic reactions of bacteriophages used in the TD and the type of solid nutrient media. The best results of phage-typing as regards the strength of lysis were obtained on agar for Brucella isolation (erythritol agar) with all bacteriophages applied in the TD. The results appeared similar to those obtained with Hottinger's medium. In order to unify the method of Staphylococcus phage-typing, it is suggested that dry erythritol agar manufactured by the Dagestan Research Institute for Nutrient Media should be used as a solid medium. In addition, two procedures for St. aureus cultivation before phage-typing are offered, raising the percentage of cultures lysed by bacteriophages.
在平行实验中评估了用于金黄色葡萄球菌噬菌体分型的八种营养培养基。对22株具有先前已知噬菌体分型公式的金黄色葡萄球菌测试菌株以及44株在食物中毒期间从不同来源分离的菌株进行了分型。噬菌体采用测试剂量（TD）和10倍TD进行使用。在所使用的噬菌体的裂解反应程度与固体营养培养基类型之间建立了明显的关系。就裂解强度而言，在用于布鲁氏菌分离的琼脂（赤藓糖醇琼脂）上使用所有噬菌体的测试剂量进行噬菌体分型时，获得了最佳结果。结果与使用霍廷格培养基获得的结果相似。为了统一葡萄球菌噬菌体分型方法，建议使用达吉斯坦营养培养基研究所生产的干燥赤藓糖醇琼脂作为固体培养基。此外，还提供了两种在噬菌体分型前培养金黄色葡萄球菌的程序，提高了被噬菌体裂解的培养物的百分比。
