Salatino Mariana, Croci Diego O, Laderach Diego J, Compagno Daniel, Gentilini Lucas, Dalotto-Moreno Tomas, Dergan-Dylon L Sebastián, Méndez-Huergo Santiago P, Toscano Marta A, Cerliani Juan P, Rabinovich Gabriel A
Laboratorio de Inmunopatología, Instituto de Biología y Medicina Experimental (IBYME), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Vuelta de Obligado 2490, C1428, Buenos Aires, Argentina,
Methods Mol Biol. 2015;1207:293-304. doi: 10.1007/978-1-4939-1396-1_19.
Formation of an aberrant and heterogeneous vascular network is a key pathological event in the multistep process of tumor growth and metastasis. Pro-angiogenic factors are synthesized and released from tumor, stromal, endothelial, and myeloid cells in response to hypoxic and immunosuppressive microenvironments which are commonly found during cancer progression. Emerging data indicate key roles for galectins, particularly galectin-1, -3, -8, and -9 in the regulation of angiogenesis in different pathophysiologic settings. Each galectin interacts with a preferred set of glycosylated receptors, triggers different signaling pathway, and promotes sprouting angiogenesis through different mechanisms. Understanding the role of galectins in tumor neovascularization will contribute to the design of novel anti-angiogenic therapies aimed at complementing current clinical approaches. Here we describe selected strategies and methods used to study the galectin-1 regulation by hypoxia and its role in blood vessel formation.
异常且异质性血管网络的形成是肿瘤生长和转移多步骤过程中的关键病理事件。促血管生成因子在肿瘤、基质、内皮和髓样细胞中合成并释放，以应对癌症进展过程中常见的缺氧和免疫抑制微环境。新出现的数据表明半乳糖凝集素，特别是半乳糖凝集素-1、-3、-8和-9在不同病理生理环境下的血管生成调节中起关键作用。每种半乳糖凝集素与一组特定的糖基化受体相互作用，触发不同的信号通路，并通过不同机制促进芽生血管生成。了解半乳糖凝集素在肿瘤新生血管形成中的作用将有助于设计旨在补充当前临床方法的新型抗血管生成疗法。在此，我们描述了用于研究缺氧对半乳糖凝集素-1的调节及其在血管形成中的作用的选定策略和方法。
