Cerri Daniel Giuliano, Arthur Connie M, Rodrigues Lílian Cataldi, Fermino Marise Lopes, Rocha Lenaldo Branco, Stowell Sean R, Baruffi Marcelo Dias
Faculty of Pharmaceutical Sciences of Ribeirão Preto, Department of Clinical, Toxicological and Bromatological Analysis, University of Sao Paulo, Ribeirão Preto-S, Brazil.
Methods Mol Biol. 2015;1207:343-54. doi: 10.1007/978-1-4939-1396-1_23.
Confocal microscopy provides a unique modality to examine the expression and localization of biomolecules in a variety of settings. Using this technique, an image is acquired from the focal plane of the objective using focused laser light, making it possible to work within the resolution limit of the optical system. In addition, by acquiring multiple images from a variety of focal planes, stacked series of images can provide clear spatial localization of a probed structure or protein. We describe herein the immunofluorescence methods for galectin staining in frozen sections of tissue for galectin localization using confocal microscopy.
共聚焦显微镜提供了一种独特的方式来检查生物分子在各种环境中的表达和定位。使用这种技术，利用聚焦激光从物镜的焦平面获取图像，从而能够在光学系统的分辨率极限内工作。此外，通过从各种焦平面获取多个图像，堆叠的图像系列可以提供被探测结构或蛋白质的清晰空间定位。我们在此描述了使用共聚焦显微镜在组织冰冻切片中进行半乳糖凝集素染色以定位半乳糖凝集素的免疫荧光方法。
