Preston Sarah, Dunphy Jillian, Beddoe Travis, Meeusen Els, Young Anna
Biotechnology Research Laboratories, School of Biomedical Sciences, Monash University, Clayton, VIC, Australia.
Methods Mol Biol. 2015;1207:371-95. doi: 10.1007/978-1-4939-1396-1_25.
Galectin-11 and galectin-14 are ruminant galectins involved in parasitic infections. Although their roles in parasite immunity are still being elucidated, its appears that their functions are parasite specific. In gastrointestinal infections with the nematode Haemonchus contortus, both galectin-11 and galectin-14 appear to be protective. However, in a chronic infection of liver fluke, Fasciola hepatica, these galectins may aid parasite survival. This chapter discusses the methods designed to study parasitic infections in sheep, which have provided us with insight into the functions of galectin-11 and galectin-14 during host-parasite interactions. These methods include parasite cultivation and infection, galectin staining of host and parasite tissue, surface staining of parasites with recombinant galectins and in vitro assays to monitor the effect of galectins on larval development.
半乳糖凝集素-11和半乳糖凝集素-14是参与寄生虫感染的反刍动物半乳糖凝集素。尽管它们在寄生虫免疫中的作用仍在阐明之中，但似乎它们的功能具有寄生虫特异性。在胃肠道受到捻转血矛线虫感染时，半乳糖凝集素-11和半乳糖凝集素-14似乎都具有保护作用。然而，在肝片吸虫的慢性感染中，这些半乳糖凝集素可能有助于寄生虫存活。本章讨论了旨在研究绵羊寄生虫感染的方法，这些方法为我们深入了解半乳糖凝集素-11和半乳糖凝集素-14在宿主-寄生虫相互作用中的功能提供了线索。这些方法包括寄生虫培养和感染、宿主和寄生虫组织的半乳糖凝集素染色、用重组半乳糖凝集素对寄生虫进行表面染色以及监测半乳糖凝集素对幼虫发育影响的体外试验。
