拉沙病毒核蛋白的核酸外切酶结构域对于避免RIG-I信号传导和抑制先天性免疫反应至关重要。
Exonuclease domain of the Lassa virus nucleoprotein is critical to avoid RIG-I signaling and to inhibit the innate immune response.
作者信息
Reynard Stéphanie, Russier Marion, Fizet Alexandra, Carnec Xavier, Baize Sylvain
机构信息
Unité de Biologie des Infections Virales Emergentes, Institut Pasteur, Lyon, France; Centre International de Recherche en Infectiologie (CIRI), Université de Lyon, INSERM U1111, Ecole Normale Supérieure de Lyon, Université Lyon 1, CNRS UMR5308, Lyon, France.
Unité de Biologie des Infections Virales Emergentes, Institut Pasteur, Lyon, France; Centre International de Recherche en Infectiologie (CIRI), Université de Lyon, INSERM U1111, Ecole Normale Supérieure de Lyon, Université Lyon 1, CNRS UMR5308, Lyon, France
出版信息
J Virol. 2014 Dec;88(23):13923-7. doi: 10.1128/JVI.01923-14. Epub 2014 Sep 24.
Abstract
Lassa virus (LASV), which causes a viral hemorrhagic fever, inhibits the innate immune response. The exonuclease (ExoN) domain of its nucleoprotein (NP) is implicated in the suppression of retinoic acid-inducible gene I (RIG-I) signaling. We show here that a LASV in which ExoN function has been abolished strongly activates innate immunity and that this effect is dependent on RIG-I signaling. These results highlight the key role of NP ExoN function in the immune evasion that occurs during LASV infection.
摘要
引起病毒性出血热的拉沙病毒（LASV）会抑制先天性免疫反应。其核蛋白（NP）的核酸外切酶（ExoN）结构域与维甲酸诱导基因I（RIG-I）信号传导的抑制有关。我们在此表明，ExoN功能已被消除的LASV会强烈激活先天性免疫，且这种效应依赖于RIG-I信号传导。这些结果突出了NP ExoN功能在LASV感染期间发生的免疫逃避中的关键作用。
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本文引用的文献
1
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PLoS Negl Trop Dis. 2014 Jan 9;8(1):e2637. doi: 10.1371/journal.pntd.0002637. eCollection 2014.
2
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J Biol Chem. 2013 Jun 7;288(23):16949-16959. doi: 10.1074/jbc.M112.420521. Epub 2013 Apr 24.
3
Cleavage of the Junin virus nucleoprotein serves a decoy function to inhibit the induction of apoptosis during infection.Junin 病毒核蛋白的裂解起到诱饵功能，以抑制感染期间细胞凋亡的诱导。
J Virol. 2013 Jan;87(1):224-33. doi: 10.1128/JVI.01929-12. Epub 2012 Oct 17.
4
Plasmacytoid dendritic cells are productively infected and activated through TLR-7 early after arenavirus infection.浆细胞样树突状细胞在沙粒病毒感染后早期通过 TLR-7 被有效感染和激活。
Cell Host Microbe. 2012 Jun 14;11(6):617-30. doi: 10.1016/j.chom.2012.04.017.
5
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J Virol. 2012 Aug;86(15):8185-97. doi: 10.1128/JVI.07240-11. Epub 2012 May 23.
6
Pathogenic Old World arenaviruses inhibit TLR2/Mal-dependent proinflammatory cytokines in vitro.旧世界致病性沙粒病毒在体外抑制 TLR2/Mal 依赖性促炎细胞因子。
J Virol. 2012 Jul;86(13):7216-26. doi: 10.1128/JVI.06508-11. Epub 2012 Apr 24.
7
Lassa virus nucleoprotein mutants generated by reverse genetics induce a robust type I interferon response in human dendritic cells and macrophages.反向遗传学生成的拉沙病毒核蛋白突变体能在人树突状细胞和巨噬细胞中诱导强烈的 I 型干扰素反应。
J Virol. 2011 Nov;85(22):12093-7. doi: 10.1128/JVI.00429-11. Epub 2011 Aug 31.
8
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