Ziegler B, Lucke S, Besch W, Hahn H J
Central Institute of Diabetes Gerhardt Katsch, Karlsburg, GDR.
Exp Clin Endocrinol. 1989 Apr;93(1):1-10. doi: 10.1055/s-0029-1210830.
The effect of a single subdiabetogenic dose of streptozotocin (30 mg/kg body weight) on pancreatic insulin content and relative beta cell volume has been studied in normoglycaemic Wistar rats treated with streptozotocin either 2, 3 or 14 days after STZ was given. A single intravenous injection of streptozotocin caused a significant reduction of pancreatic insulin content, islet and beta cell volume, accompanied by a significantly diminished islet insulin content. The glucose- and 3-isobutyl-1-methylxanthine-stimulated insulin secretion was significantly lower in islets obtained 2 or 3 days after streptozotocin injection compared with those of vehicle-treated controls. 14 days after streptozotocin injection, beta cell volume and pancreatic insulin content partially recovered. At this time islet insulin content and secretory responsiveness were enhanced compared to the early phase following streptozotocin administration. The incorporation of [3H] thymidine into islet DNA was significantly enhanced at day 2 or 3 after streptozotocin application, whereas at day 14 the DNA synthesis corresponded to values from control rats. Despite persisting normoglycaemia, the beta cell volume of streptozotocin-treated rats was only 52% vs. control values, thus indicating the unresponsiveness of residual beta cells to compensate spontaneously for the beta cell loss.
在给予链脲佐菌素（STZ）后2天、3天或14天，对正常血糖的Wistar大鼠进行研究，观察单次低于致糖尿病剂量的链脲佐菌素（30 mg/kg体重）对胰腺胰岛素含量和相对β细胞体积的影响。单次静脉注射链脲佐菌素导致胰腺胰岛素含量、胰岛和β细胞体积显著降低，同时胰岛胰岛素含量明显减少。与溶媒处理的对照组相比，链脲佐菌素注射后2天或3天获得的胰岛中，葡萄糖和3-异丁基-1-甲基黄嘌呤刺激的胰岛素分泌显著降低。链脲佐菌素注射14天后，β细胞体积和胰腺胰岛素含量部分恢复。此时，与链脲佐菌素给药后的早期阶段相比，胰岛胰岛素含量和分泌反应性增强。链脲佐菌素应用后第2天或第3天，[3H]胸苷掺入胰岛DNA的量显著增加，而在第14天，DNA合成与对照大鼠的值相当。尽管血糖持续正常，但链脲佐菌素处理大鼠的β细胞体积仅为对照值的52%，这表明残余β细胞无法自发补偿β细胞损失。
