Institute of Hydrobiology, Jinan University, Guangzhou 510632, China; Institute of Marine and Environmental Technology, University of Maryland Center for Environmental Science and University of Maryland Baltimore County, Baltimore, MD 21202, United States.
Institute of Marine and Environmental Technology, University of Maryland Center for Environmental Science and University of Maryland Baltimore County, Baltimore, MD 21202, United States.
Bioresour Technol. 2014 Nov;172:143-149. doi: 10.1016/j.biortech.2014.09.003. Epub 2014 Sep 10.
Energy-intensive chemical conversion of crude algal oils into biodiesel is a major barrier for cost-effective algal biofuel production. To overcome this problem, we developed an enzyme-based platform for conversion of crude algal oils into fatty acid methyl esters. Crude algal oils were extracted from the oleaginous microalga Nannochloropsis oceanica IMET1 and converted by an immobilized lipase from Candida antarctica. The effects of different acyl acceptors, t-butanol as a co-solvent, oil to t-butanol ratio, oil to methanol ratio, temperature and reaction time on biodiesel conversion efficiency were studied. The conversion efficiency reached 99.1% when the conversion conditions were optimized, i.e., an oil to t-butanol weight ratio of 1:1, an oil to methanol molar ratio of 1:12, and a reaction time of 4h at 25°C. The enzymatic conversion process developed in this study may hold a promise for low energy consumption, low wastewater-discharge biochemical conversion of algal feedstocks into biofuels.
从粗藻油到生物柴油的能源密集型化学转化是降低成本的藻类生物燃料生产的主要障碍。为了克服这个问题，我们开发了一种基于酶的平台，用于将粗藻油转化为脂肪酸甲酯。粗藻油从油脂微藻 Nannochloropsis oceanica IMET1 中提取，并通过来自南极假丝酵母的固定化脂肪酶进行转化。研究了不同酰基接受体、叔丁醇作为共溶剂、油与叔丁醇的比例、油与甲醇的比例、温度和反应时间对生物柴油转化率的影响。当优化转化条件，即油与叔丁醇的重量比为 1:1、油与甲醇的摩尔比为 1:12、在 25°C 下反应 4 小时时，转化率达到 99.1%。本研究中开发的酶促转化过程可能为低能耗、低废水排放的藻类原料生物转化为生物燃料提供了一种有前途的方法。
