Lin Jiajiang, He Yan, Xu Jianming, Chen Zuliang, Brookes Philip C
Institute of Soil and Water Resources and Environmental Science, Zhejiang Provincial Key Laboratory of Subtropical Soil and Plant Nutrition, Zhejiang University , Hangzhou 310058, China.
J Agric Food Chem. 2014 Oct 15;62(41):9974-81. doi: 10.1021/jf502746n. Epub 2014 Oct 6.
Vertical variations of pentachlorophenol (PCP) dissipation and microbial community were investigated in a paddy soil with the addition of electron acceptors (NO3(-), SO4(2-)) and donors (crop residues). Crop residues enhanced PCP dissipation by supplying dissolved organic carbon (DOC) as an electron donor, whereas NO3(-) and SO4(2-) inhibited it. The dissipation of PCP in electron donor treatments resulted in the accumulation of 3,4,5-trichlorophenol (3,4,5-TCP) except for wheat residues. The abundance and diversity of phospholipid fatty acids (PLFAs) decreased with increasing soil depth. The succession of predominant PLFAs shifted from aerobic bacteria to anaerobic bacteria when electron acceptors were changed to electron donors. The saturated/monounsaturated fatty acids (S/M) ratio increased with soil depth, which probably implied that nutrient turnover rate declined after the accumulation of 3,4,5-TCP. The results showed that the addition of electron donors and acceptors modified the microbial communities, which then further influenced the degradation pathway of PCP.
在添加了电子受体（NO3(-)、SO4(2-)）和供体（作物残体）的稻田土壤中，研究了五氯苯酚（PCP）降解及微生物群落的垂直变化。作物残体通过提供溶解有机碳（DOC）作为电子供体增强了PCP的降解，而NO3(-)和SO4(2-)则抑制了PCP的降解。除小麦残体外，电子供体处理中PCP的降解导致了3,4,5-三氯苯酚（3,4,5-TCP）的积累。磷脂脂肪酸（PLFA）的丰度和多样性随土壤深度增加而降低。当电子受体变为电子供体时，主要PLFA的演替从需氧细菌转变为厌氧细菌。饱和/单不饱和脂肪酸（S/M）比值随土壤深度增加而升高，这可能意味着3,4,5-TCP积累后养分周转速率下降。结果表明，电子供体和受体的添加改变了微生物群落，进而进一步影响了PCP的降解途径。
