Li Ruiping, Yuan Qin, Huang Yingping
Se Pu. 2014 Jul;32(7):675-81. doi: 10.3724/sp.j.1123.2014.04048.
Hydrophilic interaction chromatography (HILIC) is a valuable alternative to reversed phase liquid chromatography (RPLC) for the analysis of highly polar and hydrophilic compounds, in which the separation mechanism is quite different from RPLC and the separation selectivity is complementary to RPLC. This separation mode can be characterized as normal phase liquid chromatography (NPLC) on polar columns in aqueous-organic mobile phases rich in organic solvents (usually acetonitrile). Silica has been the earliest developed and most widely used HILIC stationary phase. This review deals with the recent advances in the development of the retention mechanism on silica column with special attention to the effects of stationary phase, mobile phase composition and temperature on separation in HILIC mode. Moreover, the developing trends and applications of this HILIC mode are presented.
亲水作用色谱法（HILIC）是用于分析高极性和亲水性化合物的反相液相色谱法（RPLC）的一种有价值的替代方法，其分离机制与RPLC有很大不同，且分离选择性与RPLC互补。这种分离模式可被描述为在富含有机溶剂（通常为乙腈）的水-有机流动相中的极性柱上进行的正相液相色谱法（NPLC）。硅胶是最早开发且应用最广泛的HILIC固定相。本文综述了硅胶柱保留机制发展的最新进展，特别关注固定相、流动相组成和温度对HILIC模式下分离的影响。此外，还介绍了这种HILIC模式的发展趋势和应用。
