Bao Xiao-Li, Yuan Hui-Hui, Wang Cheng-Zhong, Fan Wei, Lan Min-Bo
Shanghai Key Laboratory of Functional Materials Chemistry, and Research Centre of Analysis and Test, East China University of Science and Technology , Shanghai , PR China and.
Pharm Biol. 2015 Jan;53(1):117-24. doi: 10.3109/13880209.2014.911921. Epub 2014 Sep 26.
Abstract Context: Most of the present studies on the antitumor efficiency of Cymbopogon citratus (DC.) Stapf (Gramineae) are limited to its low-mass compounds, and little information about the antitumor activity of polysaccharides from this plant is available.
This study focused on the potential antitumor and immunomodulatory activities of polysaccharides (CCPS) from C. citratus.
CCPS was isolated using the water extraction-ethanol precipitation method. The sarcoma 180 (S180) cells-inoculated mice were intraperitoneally administrated with CCPS (30-200 mg/kg/d) for seven consecutive days. The effects of CCPS on tumor growth, thymus and spleen weights, splenocyte proliferation, and cytokine secretion in the tumor-bearing mice were measured. The cytotoxicity of CCPS (50-800 μg/mL) towards S180 cells was also studied.
CCPS significantly inhibited the growth of the transplanted S180 tumors, with the inhibition rates ranging from 14.8 to 37.8%. Simultaneously, CCPS dose-dependently improved the immunity of the tumor-bearing mice. With the highest dose of 200 mg/kg/d, the thymus and spleen indices were increased by 21.9 and 91.9%, respectively; ConA- and LSP-induced splenocyte proliferations were increased by 32.7 and 35.3%, respectively. The secretions of interleukin 2 (IL-2), interleukin 6 (IL-6), interleukin 2 (IL-12), and tumor necrosis factor-α (TNF-α) were increased by 103.2, 40.2, 23.6, and 26.3%, respectively. Nevertheless, almost no toxicity of CCPS towards S180 cells was observed, with the maximal inhibition rate less than 15% at the CCPS concentration of 800 μg/mL.
CCPS exhibited antitumor activity in vivo, and this activity might be achieved by immunoenhancement rather than direct cytotoxicity.
摘要 背景：目前大多数关于柠檬香茅（禾本科）抗肿瘤效果的研究仅限于其低质量化合物，关于该植物多糖抗肿瘤活性的信息很少。
本研究聚焦于柠檬香茅多糖（CCPS）的潜在抗肿瘤和免疫调节活性。
采用水提取-乙醇沉淀法分离CCPS。给接种肉瘤180（S180）细胞的小鼠连续7天腹腔注射CCPS（30 - 200mg/kg/d）。测定CCPS对荷瘤小鼠肿瘤生长、胸腺和脾脏重量、脾细胞增殖及细胞因子分泌的影响。还研究了CCPS（50 - 800μg/mL）对S180细胞的细胞毒性。
CCPS显著抑制移植性S180肿瘤的生长，抑制率在14.8%至37.8%之间。同时，CCPS剂量依赖性地提高荷瘤小鼠的免疫力。最高剂量200mg/kg/d时，胸腺和脾脏指数分别增加21.9%和91.9%；刀豆蛋白A（ConA）和脂多糖（LSP）诱导的脾细胞增殖分别增加32.7%和35.3%。白细胞介素2（IL - 2）、白细胞介素6（IL - 6）、白细胞介素12（IL - 12）和肿瘤坏死因子-α（TNF - α）的分泌分别增加103.2%、40.2%、23.6%和26.3%。然而，几乎未观察到CCPS对S180细胞的毒性，在CCPS浓度为800μg/mL时最大抑制率小于15%。
CCPS在体内表现出抗肿瘤活性，且该活性可能通过免疫增强而非直接细胞毒性实现。
