Yoshida F, Matsuo S, Hiramatsu T, Okura T, Yuzawa Y, Watanabe Y, Sakamoto N, Matsuo S
Nihon Jinzo Gakkai Shi. 1989 Feb;31(2):135-43.
Kidneys of Balb/c mice intravenously injected with rabbit antibodies to heparan sulfate proteoglycan (HSPG) derived from PYS-2 cells were studied for 14 weeks. Antibodies were found to bind to the glomerular basement membrane (GBM) as early as 15 min after the injection. Binding of antibodies was observed over the whole thickness of the GBM. The lamina rara interna and foot process base appeared to be more intensely stained. This pattern did not change throughout the experiments. Mild inflammatory changes (infiltration of polymorphonuclear cells and swelling of the glomerular endothelium) were seen at the initial stage (approximately 1 day), and mesangial expansion followed (1-4 weeks). In mice pre- and booster-immunized with normal rabbit IgG, a moderate autologous response was observed by immunofluorescence microscopy, but no significant inflammatory changes were noted. At the late stage (6 weeks approximately), irregular GBM thickening was observed. The thickening was due mainly to expansion of the lamina rara externa. These findings suggest that the anti-HSPG antibodies caused mild glomerulonephritis at the initial stage, and later caused thickening of the GBM possibly by disturbing the assembly, production and degradation of GBM components.
对静脉注射源自PYS-2细胞的硫酸乙酰肝素蛋白聚糖（HSPG）兔抗体的Balb/c小鼠肾脏进行了14周的研究。发现抗体在注射后15分钟就最早与肾小球基底膜（GBM）结合。在GBM的整个厚度上都观察到了抗体的结合。内疏松层和足突基部似乎染色更强烈。在整个实验过程中这种模式没有改变。在初始阶段（大约1天）可见轻度炎症变化（多形核细胞浸润和肾小球内皮肿胀），随后是系膜扩张（1 - 4周）。在用正常兔IgG进行预免疫和加强免疫的小鼠中，通过免疫荧光显微镜观察到适度的自体反应，但未发现明显的炎症变化。在后期（大约6周），观察到GBM不规则增厚。增厚主要是由于外疏松层的扩张。这些发现表明，抗HSPG抗体在初始阶段引起轻度肾小球肾炎，后期可能通过干扰GBM成分的组装、产生和降解导致GBM增厚。
