Seo Min-Ju, Shin Kyung-Chul, Jeong Yeon-Ju, Oh Deok-Kun
Department of Bioscience and Biotechnology, Konkuk University, 1 Hwayang-dong, Gwangjin-Gu, Seoul, 143-701, Republic of Korea.
Biotechnol Lett. 2015 Jan;37(1):131-7. doi: 10.1007/s10529-014-1650-y. Epub 2014 Sep 26.
The optimal conditions for the production of 5,8-dihydroxy-9(Z)-octadecenoic acid (5,8-diHOME) from oleic acid by whole recombinant Escherichia coli cells expressing diol synthase from Aspergillus nidulans were 40 °C, pH 7.5, 10 % (v/v) dimethyl sulfoxide, 35 g cells l(-1), and 12 g oleic acid l(-1) at 250 rpm in a 250 ml baffled flask. Under these conditions, whole recombinant cells produced 5.2 g 5,8-diHOME l(-1) together with 1 g l(-1) of the intermediate 8-hydroperoxy-9(Z)-octadecenoic acid (8-HPOME) after 60 min. This corresponded to a conversion yield of 43 % (w/w), a volumetric productivity of 5.2 g l(-1 )h(-1), and a specific productivity of 148 mg g cells(-1 )h(-1). This is the first report of the biotechnological production of 5,8-diHOME from oleic acid.
利用表达来自构巢曲霉二醇合酶的全重组大肠杆菌细胞，由油酸生产5,8 - 二羟基-9(Z)-十八碳烯酸（5,8 - 二羟基十八碳烯酸，5,8 - diHOME）的最佳条件为：在250 ml带挡板的烧瓶中，40℃、pH 7.5、10%（v/v）二甲基亚砜、35 g细胞l⁻¹、12 g油酸l⁻¹，转速250 rpm。在此条件下，全重组细胞在60分钟后产生了5.2 g 5,8 - diHOME l⁻¹以及1 g l⁻¹的中间产物8 - 氢过氧基-9(Z)-十八碳烯酸（8 - HPOME）。这相当于转化率为43%（w/w），体积产率为5.2 g l⁻¹ h⁻¹，比产率为148 mg g细胞⁻¹ h⁻¹。这是关于从油酸生物技术生产5,8 - diHOME的首次报道。
