Gao Jie, Xue Jun-Fa, Xu Meng, Gui Bao-Song, Wang Feng-Xin, Ouyang Jian-Ming
Department of Nephrology, the Second Hospital of Xi'an Jiaotong University, Xi'an, People's Republic of China.
Institute of Biomineralization and Lithiasis Research, Jinan University, Guangzhou, People's Republic of China.
Int J Nanomedicine. 2014 Sep 16;9:4399-409. doi: 10.2147/IJN.S66000. eCollection 2014.
This study aimed to accurately analyze the relationship between calcium oxalate (CaOx) stone formation and the components of urinary nanocrystallites.
High-resolution transmission electron microscopy (HRTEM), selected area electron diffraction, fast Fourier transformation of HRTEM, and energy dispersive X-ray spectroscopy were performed to analyze the components of these nanocrystallites.
The main components of CaOx stones are calcium oxalate monohydrate and a small amount of dehydrate, while those of urinary nanocrystallites are calcium oxalate monohydrate, uric acid, and calcium phosphate. The mechanism of formation of CaOx stones was discussed based on the components of urinary nanocrystallites.
The formation of CaOx stones is closely related both to the properties of urinary nanocrystallites and to the urinary components. The combination of HRTEM, fast Fourier transformation, selected area electron diffraction, and energy dispersive X-ray spectroscopy could be accurately performed to analyze the components of single urinary nanocrystallites. This result provides evidence for nanouric acid and/or nanocalcium phosphate crystallites as the central nidus to induce CaOx stone formation.
本研究旨在准确分析草酸钙（CaOx）结石形成与尿液纳米晶体成分之间的关系。
采用高分辨率透射电子显微镜（HRTEM）、选区电子衍射、HRTEM的快速傅里叶变换以及能量色散X射线光谱法来分析这些纳米晶体的成分。
CaOx结石的主要成分是一水草酸钙和少量二水草酸钙，而尿液纳米晶体的主要成分是一水草酸钙、尿酸和磷酸钙。基于尿液纳米晶体的成分对CaOx结石的形成机制进行了探讨。
CaOx结石的形成与尿液纳米晶体的性质以及尿液成分密切相关。结合HRTEM、快速傅里叶变换、选区电子衍射和能量色散X射线光谱法能够准确地分析单个尿液纳米晶体的成分。该结果为纳米尿酸和/或纳米磷酸钙晶体作为诱导CaOx结石形成的核心病灶提供了证据。
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