Anders M, Matthäus W
Cesk Oftalmol. 1989 May;45(3):162-8.
On 10 experimental animals (20 eyes of bastard rabbits) the authors tested the possibility to influence by cryostimulation the increase of the acid mucopolysaccharide content (glycosaminoglycans or proteoglycans) after damaging the eye with 6.0% = 1.4 N sulphuric acid. Eight corneae were subjected to cryotherapeutic treatment 4-6 hours after corrosion, by the method of contact freezing with a cryoapparatus OKG 2, using a technique which in the first part of the work had a convincing cryostimulating effect: working temperature -70 degrees C, 10 spots with a diameter of 2 mm for 7 seconds placed directly in the cornea surrounding the damaged area. As controls for both groups (corroded and cryotreated and corroded not treated) served four normal intact corneae (two animals as above). Collection of samples of the cornea was made on the 4th and 10th day after corrosion. The corneae were processed by histological methods stained with toluidine and alcian blue. It was revealed that on the 4th as well as 10th day after cryostimulation the acid mucopolysaccharide content was higher than without cryostimulation. On the 10th day after the injury and cryotherapy the acid mucopolysaccharide content was normal. The results were evaluated by Fischer's test. The increase of acid mucopolysaccharides on the cornae was more rapid than at the 1% level of significance, as compared with corneae without cryotherapy. Cryotherapy thus promoted restoration of acid mucopolysaccharides in the corroded corneae.
作者在10只实验动物（杂种兔的20只眼睛）上测试了在6.0% = 1.4N硫酸损伤眼睛后，通过冷冻刺激影响酸性粘多糖含量（糖胺聚糖或蛋白聚糖）增加的可能性。8只角膜在腐蚀后4 - 6小时接受冷冻治疗，采用OKG 2冷冻装置接触冷冻法，使用在研究第一部分具有令人信服的冷冻刺激效果的技术：工作温度为 - 70℃，在受损区域周围的角膜上直接放置10个直径为2mm的点，持续7秒。两组（接受冷冻治疗的腐蚀组和未接受治疗的腐蚀组）的对照为4只正常完整的角膜（上述两只动物）。在腐蚀后的第4天和第10天采集角膜样本。角膜样本采用甲苯胺和阿尔辛蓝染色的组织学方法处理。结果显示，在冷冻刺激后的第4天和第10天，酸性粘多糖含量高于未进行冷冻刺激的情况。在损伤和冷冻治疗后的第10天，酸性粘多糖含量恢复正常。结果通过费舍尔检验进行评估。与未接受冷冻治疗的角膜相比，角膜上酸性粘多糖的增加在1%显著水平上更快。因此，冷冻治疗促进了腐蚀角膜中酸性粘多糖的恢复。
