Rambla-Alegre Maria, Tienpont Bart, Mitsui Kazuhisa, Masugi Eri, Yoshimura Yuta, Nagata Hisanori, David Frank, Sandra Pat
Research Institute for Chromatography, President Kennedypark 26, B-8500 Kortrijk, Belgium.
Research Institute for Chromatography, President Kennedypark 26, B-8500 Kortrijk, Belgium.
J Chromatogr A. 2014 Oct 24;1365:191-203. doi: 10.1016/j.chroma.2014.09.015. Epub 2014 Sep 16.
Aroma characterization of whole cigarette smoke samples using sensory panels or electronic nose (E-nose) devices is difficult due to the masking effect of major constituents and solvent used for the extraction step. On the other hand, GC in combination with olfactometry detection does not allow to study the delicate balance and synergetic effect of aroma solutes. To overcome these limitations a new instrumental set-up consisting of heart-cutting gas chromatography using a capillary flow technology based Deans switch and low thermal mass GC in combination with an electronic nose device is presented as an alternative to GC-olfactometry. This new hyphenated GC-E-nose configuration is used for the characterization of cigarette smoke aroma. The system allows the transfer, combination or omission of selected GC fractions before injection in the E-nose. Principal component analysis (PCA) and discriminant factor analysis (DFA) allowed clear visualizing of the differences among cigarette brands and classifying them independently of their nicotine content. Omission and perceptual interaction tests could also be carried out using this configuration. The results are promising and suggest that the GC-E-nose hyphenation is a good approach to measure the contribution level of individual compounds to the whole cigarette smoke.
由于主要成分的掩盖效应以及萃取步骤中使用的溶剂，利用感官小组或电子鼻设备对完整卷烟烟雾样品进行香气表征很困难。另一方面，气相色谱结合嗅觉检测无法研究香气溶质之间微妙的平衡和协同效应。为克服这些限制，提出了一种新的仪器装置，该装置由采用基于毛细管流技术的迪恩斯切换器的中心切割气相色谱和低热质量气相色谱与电子鼻设备组合而成，作为气相色谱 - 嗅觉检测的替代方法。这种新的联用气相色谱 - 电子鼻配置用于表征卷烟烟雾香气。该系统允许在注入电子鼻之前对选定的气相色谱馏分进行转移、合并或舍弃。主成分分析（PCA）和判别因子分析（DFA）能够清晰地呈现卷烟品牌之间的差异，并独立于尼古丁含量对其进行分类。使用这种配置还可以进行舍弃和感知相互作用测试。结果很有前景，表明气相色谱 - 电子鼻联用是衡量单个化合物对整个卷烟烟雾贡献水平的一种好方法。
