State Key Laboratory of Cotton Biology, Key Laboratory of Biological and Genetic Breeding of Cotton, the Ministry of Agriculture, Institute of Cotton Research, the Chinese Academy of Agricultural Sciences, Anyang, 455000, China.
J Integr Plant Biol. 2015 May;57(5):450-67. doi: 10.1111/jipb.12288. Epub 2014 Nov 24.
To introgress the good fiber quality and yield from Gossypium barbadense into a commercial Upland cotton variety, a high-density simple sequence repeat (SSR) genetic linkage map was developed from a BC1 F1 population of Gossypium hirsutum × Gossypium barbadense. The map comprised 2,292 loci and covered 5115.16 centiMorgan (cM) of the cotton AD genome, with an average marker interval of 2.23 cM. Of the marker order for 1,577 common loci on this new map, 90.36% agrees well with the marker order on the D genome sequence genetic map. Compared with five published high-density SSR genetic maps, 53.14% of marker loci were newly discovered in this map. Twenty-six quantitative trait loci (QTLs) for lint percentage (LP) were identified on nine chromosomes. Nine stable or common QTLs could be used for marker-assisted selection. Fifty percent of the QTLs were from G. barbadense and increased LP by 1.07%-2.41%. These results indicated that the map could be used for screening chromosome substitution segments from G. barbadense in the Upland cotton background, identifying QTLs or genes from G. barbadense, and further developing the gene pyramiding effect for improving fiber yield and quality.
为了将海岛棉的优良纤维品质和产量导入到商业陆地棉品种中，我们利用陆地棉×海岛棉的 BC1F1 群体构建了一张高密度的简单重复序列（SSR）遗传连锁图谱。该图谱包含 2292 个标记，覆盖了棉花 A 基因组的 5115.16cM，平均标记间距离为 2.23cM。在新图谱的 1577 个共有标记中，有 90.36%与 D 基因组序列遗传图谱上的标记顺序一致。与五个已发表的高密度 SSR 遗传图谱相比，该图谱中新发现的标记位点有 53.14%。在九个染色体上共鉴定到 26 个纤维百分率（LP）的数量性状位点（QTL）。九个稳定或共同的 QTL 可用于标记辅助选择。50%的 QTL 来自海岛棉，可将 LP 提高 1.07%-2.41%。这些结果表明，该图谱可用于筛选陆地棉背景下的海岛棉染色体替代片段，鉴定来自海岛棉的 QTL 或基因，并进一步开发基因聚合效应以提高纤维产量和品质。
