Pan Y, Cui Y, Yu S, Zhang Q, Fan J, Abdul Rasheed B, Yang K
Gansu Province Livestock Embryo Engineering Research Center, College of Veterinary Medicine, Gansu Agricultural University, Lanzhou, China.
Reprod Domest Anim. 2014 Dec;49(6):970-6. doi: 10.1111/rda.12416. Epub 2014 Sep 26.
Growth factors play critical role in cell proliferation, regulate tissue differentiation and modulate organogenesis. Several growth factors have been identified in the testes of various mammalian species in last few years. In present investigation, the objective was to determine the expression of epidermal growth factor (EGF) and the epidermal growth factor receptor (EGFR) in yak testicular tissue by relative quantitative real time polymerase chain reaction (RT-PCR), Western blot (WB) and immunohistochemistry (IHC) from mRNA and protein levels. The testicular tissues were collected from male yak at 6 and 24 months old. Results of RT-PCR and WB showed that the expression quantity of EGF and EGFR at 24 months of age was higher than at 6 months, and the increase rate of EGFR on mRNA and protein levels was higher than the increase rate EGF during post-natal testes development. Positive staining for EGF and EGFR was very low and mainly localized to Leydig cells testes at 6 months of age with immunohistochemistry, and seminiferous tubules were not observed. At 24 month of age, both the EGF and EGFR could be detected in Leydig cells, peritubular myoid cells, sertoli cells and germ cells of the yak testes. However, EGF and EGFR were localized to preferential adluminal compartment and basal compartment in the seminiferous tubules, respectively. In conclusion, the findings in present studies suggest that EGF and EGFR as important paracrine and/or autocrine regulators in yak testes development and spermatogenesis.
生长因子在细胞增殖中起关键作用，调节组织分化并调控器官形成。在过去几年中，已在多种哺乳动物的睾丸中鉴定出几种生长因子。在本研究中，目的是通过相对定量实时聚合酶链反应（RT-PCR）、蛋白质印迹法（WB）和免疫组织化学（IHC），从mRNA和蛋白质水平确定牦牛睾丸组织中表皮生长因子（EGF）和表皮生长因子受体（EGFR）的表达。从6月龄和24月龄的雄性牦牛采集睾丸组织。RT-PCR和WB结果显示，24月龄时EGF和EGFR的表达量高于6月龄，且在出生后睾丸发育过程中，EGFR在mRNA和蛋白质水平的增加速率高于EGF。免疫组织化学显示，6月龄时EGF和EGFR的阳性染色非常低，主要定位于睾丸间质细胞，未观察到生精小管。在24月龄时，牦牛睾丸的间质细胞、睾丸肌样细胞、支持细胞和生殖细胞中均可检测到EGF和EGFR。然而，EGF和EGFR分别定位于生精小管中的近腔室和基底部。总之，本研究结果表明，EGF和EGFR是牦牛睾丸发育和精子发生中重要的旁分泌和/或自分泌调节因子。
