[Carbonic anhydrase III and mRNA expression levels in quadriceps femoris muscle of chronic obstructive pulmonary disease patients].

OBJECTIVE

To explore the expression status of carbonic anhydrase III (CAIII) from quadriceps femoris muscle in two kinds of muscle clinical phenotype (skeletal muscle atrophy group and skeletal muscles non-atrophy group) of chronic obstructive pulmonary disease (COPD).

METHODS

Totally 37 inpatients from our hospital, were divided into 11 patients without COPD and 26 patients with COPD, in addition, according to body mass index, fat free mass index and quadriceps cross-section diameter, patients with COPD were divided into 14 skeletal muscles non-atrophy patients (SMNA) and 12 skeletal muscle atrophy patients (SMA). CAIII concentration of femoris quadriceps specimens was quantitatively determined using Western blot methods, CAIIImRNA expression levels of femoris quadriceps specimens were also quantitatively measured using RT-PCR, then compared among the 3 groups.

RESULTS

There was significant difference in CAIII quantitative concentration and CAIIImRNA expression level in each group (P < 0.05) , further more, CAIII concentration expression level was significantly higher (P < 0.01) in SMA group (1.260 ± 0.068) than in SMNA group (1.110 ± 0.014) , the latter was significantly higher (P < 0.01) than in the control group (1.000 ± 0.062) . CAIIImRNA expression level was significantly higher (P < 0.01) in SMNA group (2.170 ± 0.412) than in the control group (1.000 ± 0.115) , and was significantly lower than in SMA group (3.770 ± 0.788; P < 0.01). CAIII concentration and CAIIImRNA expression level increased at equal pace in SMNA group and SMA group, however, CAIII quantitative concentration and CAIIImRNA expression level were inconsistent in the two groups.

CONCLUSION

The expression status of CAIII in quadriceps femoris muscle was different in two kinds of muscle clinical phenotype of COPD.