Wei Haiyan, Tan Kehong, Sun Rongli, Yin Lihong, Zhang Juan, Pu Yuepu
Key Laboratory of Environmental Medicine Engineering of Ministry of Education, School of Public Health, Southeast University, Nanjing 210009, China.
Int J Environ Res Public Health. 2014 Sep 26;11(10):10036-50. doi: 10.3390/ijerph111010036.
Previous studies have shown that formaldehyde (FA) could cause immunotoxicity by changing the number of T lymphocytes and that cytokines play a pivotal role in the regulation of T lymphocytes. However, the previously used cytokine detection methods are difficult to use in the measurement of several cytokines in a small amount of sample for one test. Therefore, the cytometric bead array (CBA) technique was used. CBA showed better analytical efficiency and sensitivity than the previous methods. C57BL/6 mice were exposed to the control (normal saline), low FA concentration (0.5 mg/kg), and high FA concentration (2 mg/kg) for 1 week or 1 month. The contents of cytokines, including Th1-related cytokines (IL-2, IFN-γ, and tumor necrosis factor), Th2-related cytokines (IL-4, IL-6, and IL-10), and Th17-related cytokines (IL-17A), were measured by using the BD FACS Canto II Flow Cytometer and analyzed by FCAP ArrayTM Software. Th1/Th2/Th17-related cytokines showed a slightly decreasing trend after low FA exposure. Conversely, a significantly increasing trend was found after high FA exposure. Th1/Th2/Th17-related cytokines all serve important functions in the immune reactions in mice after FA exposure.
先前的研究表明，甲醛（FA）可通过改变T淋巴细胞数量引起免疫毒性，且细胞因子在T淋巴细胞的调节中起关键作用。然而，先前使用的细胞因子检测方法难以用于在一次检测中对少量样本中的多种细胞因子进行测量。因此，采用了细胞计数珠阵列（CBA）技术。CBA显示出比先前方法更好的分析效率和灵敏度。将C57BL/6小鼠暴露于对照（生理盐水）、低FA浓度（0.5 mg/kg）和高FA浓度（2 mg/kg）下1周或1个月。使用BD FACS Canto II流式细胞仪测量包括Th1相关细胞因子（IL-2、IFN-γ和肿瘤坏死因子）、Th2相关细胞因子（IL-4、IL-6和IL-10）以及Th17相关细胞因子（IL-17A）在内的细胞因子含量，并通过FCAP ArrayTM软件进行分析。低剂量FA暴露后，Th1/Th2/Th17相关细胞因子呈轻微下降趋势。相反，高剂量FA暴露后则呈现出显著上升趋势。Th1/Th2/Th17相关细胞因子在FA暴露后小鼠的免疫反应中均发挥重要作用。
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