Molecular cloning and expression of human leukotriene A4 hydrolase cDNA.
作者信息
Minami M, Minami Y, Ohno S, Suzuki K, Ohishi N, Shimizu T, Seyama Y
机构信息
Department of Physiological Chemistry and Nutrition, Faculty of Medicine, University of Tokyo, Japan.
出版信息
Adv Prostaglandin Thromboxane Leukot Res. 1989;19:478-83.
PMID:2526522
Abstract
We have isolated a near full-length cDNA encoding human leukotriene A4 (LTA4) hydrolase from a human spleen cDNA library. The mature form of the enzyme consists of 610 amino acid residues and its molecular weight is calculated to be 69,153. The cDNA was inserted into an expression vector (pUC 9) and expressed in Escherichia coli. The fusion protein (EX85) possessed the LTA4 hydrolase activity with kinetic properties similar to the native enzyme.
摘要
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