Pawelec G, Schmidt H, Rehbein A, Busch F
Immunology Laboratory, Medizinische Klinik, Tübingen, Federal Republik of Germany.
Cancer Immunol Immunother. 1989;29(4):242-6. doi: 10.1007/BF00199211.
Cytosine arabinoside (Ara-C) treatment of peripheral blood mononuclear cells from 12/12 chronic-phase chronic myelogenous leukaemia (CML) patients revealed a proliferative response stimulated by their untreated leukaemic cells. Specific recognition of tumour cells by patients' normal lymphocytes was suggested by the finding that cells of siblings genotypically identical for human leukocyte antigen caused no stimulation. Lymphocytes thus stimulated by tumour cells from one of these patients were cloned by limiting dilution and tested for antileukaemic effects in cytotoxicity and proliferation assays. Cytotoxic lines were isolated that killed autologous CML targets but only a limited number of allogeneic fresh leukaemias or cell lines. These results show that anti-leukaemia effectors can be isolated from chronic-phase CML patients and suggest their potential application in adoptive immunotherapy.
用阿糖胞苷(Ara-C)处理12例处于慢性期的慢性粒细胞白血病(CML)患者的外周血单个核细胞,结果显示其未处理的白血病细胞可刺激细胞增殖。同基因人类白细胞抗原的同胞的细胞未引起刺激,这一发现提示患者的正常淋巴细胞对肿瘤细胞具有特异性识别。通过有限稀释法克隆了其中一名患者的肿瘤细胞刺激的淋巴细胞,并在细胞毒性和增殖试验中检测其抗白血病作用。分离出了能杀伤自体CML靶细胞但仅能杀伤有限数量的同种异体新鲜白血病细胞或细胞系的细胞毒性细胞系。这些结果表明,可从慢性期CML患者中分离出抗白血病效应细胞,并提示其在过继性免疫治疗中的潜在应用。