Panayotides P, Porwit A, Sjögren A M, Wasserman J, Reizenstein P
Division of Hematology, Karolinska Hospital, Stockholm, Sweden.
Eur J Haematol. 1988 Apr;40(4):362-7. doi: 10.1111/j.1600-0609.1988.tb00192.x.
Peripheral blood mononuclear cells (PBMC) from healthy donors and AML patients in remission were stimulated with phytohemagglutinin (PHA) and recombinant interleukin-2 (IL-2). These stimulated cells (lymphokine activated killer (LAK) cells) showed increased DNA synthesis as measured by 3H-Thymidine uptake. A synergistic effect of PHA and IL-2 was found. LAK cells' ability to kill acute myeloid leukemia (AML) blasts was investigated by the 51Cr release assay. LAK cells showed a cytotoxicity (over 10% specific 51Cr release) against 9/12 leukemic blasts, even at effector/target (E/T) ratios as low as 5:1. However, on average only 22.2% (SD 11.8) and 36.5% (SD 12.5) 51Cr release were obtained in 4- and 18-hour cytotoxicity assays, respectively, at an E/T ratio of 20:1. Leukemic blasts in 3/12 AML cases and normal PBMC were entirely resistant to lysis, even at an E/T ratio of 80:1. Susceptibility to lysis was not correlated to peanut-agglutinin receptor expression. LAK cells were more cytotoxic towards the K-562 cell line (natural killer activity) than unstimulated PBMC.
用植物血凝素（PHA）和重组白细胞介素-2（IL-2）刺激来自健康供体和处于缓解期的急性髓系白血病（AML）患者的外周血单个核细胞（PBMC）。通过³H-胸腺嘧啶核苷摄取量测定，这些被刺激的细胞（淋巴因子激活的杀伤细胞，即LAK细胞）显示出DNA合成增加。发现PHA和IL-2有协同作用。通过⁵¹Cr释放试验研究了LAK细胞杀伤急性髓系白血病（AML）原始细胞的能力。LAK细胞对9/12例白血病原始细胞显示出细胞毒性（特异性⁵¹Cr释放超过10%），即使效应细胞/靶细胞（E/T）比例低至5:1。然而，在E/T比例为20:1时，在4小时和18小时细胞毒性试验中，平均分别仅获得22.2%（标准差11.8）和36.5%（标准差12.5）的⁵¹Cr释放。在3/12例AML病例中的白血病原始细胞和正常PBMC即使在E/T比例为80:1时也完全抵抗裂解。对裂解的敏感性与花生凝集素受体表达无关。与未刺激的PBMC相比，LAK细胞对K-562细胞系的细胞毒性更强（自然杀伤活性）。
