Bankhurst A D, Imir T
Department of Medicine, University of New Mexico, School of Medicine, Albuquerque 87131.
Cell Immunol. 1989 Aug;122(1):108-21. doi: 10.1016/0008-8749(89)90152-4.
The induction of enhanced natural cytotoxicity from human peripheral mononuclear cells by staphylococcal enterotoxin B (SEB) was examined. The activated killer cytotoxicity (AKC) was maximum at 16 hr with 1 mg/ml SEB. The precursor and effector cells of AKC were determined to be primarily CD5 negative, CD8 negative, CD16 positive cells. Monocytes and interleukin-1 played no role in the generation of AKC. However, a major role for interleukin-2 (IL-2) in AKC was shown by the inhibition of AKC when anti-IL-2 antibody or cyclosporin was added to the induction cultures. SEB rapidly induced the production of IL-2 from glass nonadherent cells by 6 hr and reached peak levels by 24 hr (162 U/ml). IL-2 induced by SEB in these induction cultures was preferentially produced by CD16 positive cells. Even though interferon-gamma (IFN-gamma) production was induced in these cultures, no role for IFN could be shown in SEB-induced AKC.
研究了葡萄球菌肠毒素B（SEB）对人外周血单个核细胞增强自然细胞毒性的诱导作用。用1mg/ml SEB诱导时，活化杀伤细胞毒性（AKC）在16小时达到最大值。AKC的前体细胞和效应细胞主要为CD5阴性、CD8阴性、CD16阳性细胞。单核细胞和白细胞介素-1在AKC的产生中不起作用。然而，当在诱导培养物中加入抗白细胞介素-2抗体或环孢素时，白细胞介素-2（IL-2）在AKC中起主要作用，这表明了其对AKC的抑制作用。SEB在6小时内迅速诱导玻璃非贴壁细胞产生IL-2，并在24小时达到峰值水平（162U/ml）。SEB在这些诱导培养物中诱导产生的IL-2优先由CD16阳性细胞产生。尽管在这些培养物中诱导产生了干扰素-γ（IFN-γ），但在SEB诱导的AKC中未显示出IFN的作用。
