Gimenez Gabriela Gregolin, Ruiz Suelen Pereira, Caetano Wilker, Peralta Rosane Marina, Matioli Graciette
Postgraduate Program in Pharmaceutical Science, State University of Maringá (UEM), Av. Colombo, 5790, Maringá, PR, CEP 87020-900, Brazil.
World J Microbiol Biotechnol. 2014 Dec;30(12):3229-44. doi: 10.1007/s11274-014-1750-9. Epub 2014 Sep 30.
Lentinus edodes CCB-42 was immobilized in loofa sponges and applied to the biosorption of the synthetic dyes congo red, bordeaux red and methyl violet. Live immobilized microorganisms achieved average decolorations of congo red, bordeaux red and methyl violet of 97.8, 99.7 and 90.6 %, respectively. The loofa sponge was the support and the coadjuvant promoting dye adsorption. The biosorption conditions were optimized for each dye, yielding 30 °C, pH 5.0 and a 12 h reaction time for congo red; 25 °C, pH 3.0 and 36 h for bordeaux red; and 25 °C, pH 8.0 and 24 h for methyl violet. Operational stability was evaluated over five consecutive cycles, with both bordeaux red and congo red exhibiting decolorations above 90 %, while the decoloration of methyl violet decreased after the third cycle. In the sixth month of storage, congo red, bordeaux red and methyl violet had decolorations of 93.1, 79.4 and 73.8 %, respectively. Biosorption process best fit the pseudo-second-order kinetic and Freundlich isotherm models. Maximum biosorption capacity of heat-treated L. edodes immobilized in loofa sponge was determined as 143.678, 500.00 and 381.679 mg/g for congo red, bordeaux red and methyl violet, respectively. Treatment with immobilized L. edodes reduced the phytotoxicity of the medium containing dyes. FT-Raman experiments suggested the occurrence of interactions between loofa sponge fibers, L. edodes and dye. L. edodes CCB-42 immobilized in loofa sponges represents a promising new mode of treatment of industrial effluents.
香菇CCB - 42被固定在丝瓜海绵中，并应用于对合成染料刚果红、枣红和甲基紫的生物吸附。活的固定化微生物对刚果红、枣红和甲基紫的平均脱色率分别达到97.8%、99.7%和90.6%。丝瓜海绵作为载体和促进染料吸附的助剂。针对每种染料优化了生物吸附条件，刚果红的最佳条件为30℃、pH 5.0和12小时反应时间；枣红为25℃、pH 3.0和36小时；甲基紫为25℃、pH 8.0和24小时。连续五个循环评估了操作稳定性，枣红和刚果红的脱色率均高于90%，而甲基紫在第三个循环后脱色率下降。储存六个月时，刚果红、枣红和甲基紫的脱色率分别为93.1%、79.4%和73.8%。生物吸附过程最符合伪二级动力学和弗伦德里希等温线模型。固定在丝瓜海绵中的热处理香菇对刚果红、枣红和甲基紫的最大生物吸附容量分别测定为143.678、500.00和381.679 mg/g。用固定化香菇处理降低了含染料培养基的植物毒性。傅里叶变换拉曼光谱实验表明丝瓜海绵纤维、香菇和染料之间发生了相互作用。固定在丝瓜海绵中的香菇CCB - 42代表了一种有前途的工业废水处理新模式。
