Milwidsky A, Kaneti H, Finci Z, Laufer N, Tsafriri A, Mayer M
Department of Obstetrics and Gynecology, Hadassah University Hospital, Mount Scopus, Jerusalem, Israel.
Fertil Steril. 1989 Aug;52(2):274-80. doi: 10.1016/s0015-0282(16)60855-5.
Plasminogen activator activity was determined in human follicular fluids (FFs) obtained during in vitro fertilization procedures. The fibrinolytic activity of plasminogen activator was significantly higher in fluids from follicles that contained oocytes that were later found to fertilize in vitro (group F) as compared with fluids from follicles that contained oocytes that failed to fertilize (NF). To assess whether this difference in overt plasminogen activator activity reflects differences in conversion of an inactive, latent plasminogen activator to the active enzyme, the ability of exogenous trypsin to enhance plasminogen activation was measured. The plasminogen-dependent hydrolysis of the chromogenic substrate S-2444 in presence of trasylol (Bayer, Leverkusen, Germany) was taken as a measure of plasminogen activator activity in these experiments. No activity was found in untreated FFs, while exposure to trypsin resulted in emergence of marked plasminogen activator activity. In addition, FFs exhibited trasylol-sensitive chromogenic activity indicative of serine-protease activity. Both the plasminogen activator and serine-protease levels after tryptic activation were significantly higher in NF than in F samples. Thus, while F samples have most of their plasminogen activator in an active form, NF samples have most of their plasminogen activator in a latent, trypsin-activatable form. Follicular fluids also contain inhibitory activities toward plasmin and trypsin. The inhibition of these enzymes correlates positively with the latency of plasminogen activator. These results suggest a direct relationship between the ability of oocytes to fertilize and the overt to latent plasminogen activator activity ratios in the FFs.
在体外受精过程中获取的人卵泡液（FFs）中测定纤溶酶原激活剂活性。与含有未受精卵子的卵泡液（NF）相比，含有后来被发现可体外受精的卵子的卵泡液（F组）中纤溶酶原激活剂的纤溶活性显著更高。为了评估这种明显的纤溶酶原激活剂活性差异是否反映了无活性的潜伏性纤溶酶原激活剂向活性酶转化的差异，测定了外源性胰蛋白酶增强纤溶酶原激活的能力。在这些实验中，将在抑肽酶（德国勒沃库森拜耳公司）存在下对发色底物S - 2444的纤溶酶原依赖性水解作为纤溶酶原激活剂活性的指标。未处理的卵泡液中未发现活性，而暴露于胰蛋白酶会导致显著的纤溶酶原激活剂活性出现。此外，卵泡液表现出对抑肽酶敏感的发色活性，表明存在丝氨酸蛋白酶活性。胰蛋白酶激活后，NF中的纤溶酶原激活剂和丝氨酸蛋白酶水平均显著高于F样本。因此，虽然F样本中的大部分纤溶酶原激活剂呈活性形式，但NF样本中的大部分纤溶酶原激活剂呈潜伏的、可被胰蛋白酶激活的形式。卵泡液还含有对纤溶酶和胰蛋白酶的抑制活性。这些酶的抑制作用与纤溶酶原激活剂的潜伏性呈正相关。这些结果表明卵子受精能力与卵泡液中活性与潜伏性纤溶酶原激活剂活性比率之间存在直接关系。
