Deutinger J, Kirchheimer J C, Reinthaller A, Christ G, Tatra G, Binder B R
Second Department of Obstetrics and Gynaecology, University of Vienna, Austria.
Hum Reprod. 1988 Jul;3(5):597-9. doi: 10.1093/oxfordjournals.humrep.a136752.
An increased production of plasminogen activators, able to convert plasminogen into plasmin, has been found in experiments in vivo on rat ovarian granulosa cells at the time of ovulation, indicating an involvement in follicular rupture. The granulosa cells of 49 follicles from 20 patients undergoing in-vitro fertilization were obtained by laparoscopy and tested for the content of urokinase-type plasminogen activator (u-PA), tissue-type plasminogen activator (t-PA) and inhibitor of plasminogen activator (PAI). In the respective follicular fluids the concentrations of oestradiol (E2), progesterone (P) and testosterone (T) were determined and the levels of these enzymes and of the follicular steroid content were related to the fertilizing behaviour of the respective oocytes. Follicles containing oocytes which could be fertilized, revealed significantly higher follicular fluid E2 and P levels and significantly lower T levels than follicles with unfertilized oocytes. The respective granulosa cells of fertilized oocytes exhibited higher levels of t-PA compared to their unfertilized counterparts, whereas no significant difference occurred in the levels of u-PA and PAI. These data suggest that successful fertilization of human oocytes is associated with a high content of t-PA in granulosa cells and high E2 and P levels in the follicular fluid.
在对大鼠卵巢颗粒细胞进行的体内实验中发现，排卵时纤溶酶原激活剂的产量增加，纤溶酶原激活剂能够将纤溶酶原转化为纤溶酶，这表明其参与了卵泡破裂过程。通过腹腔镜检查从20名接受体外受精的患者的49个卵泡中获取颗粒细胞，并检测尿激酶型纤溶酶原激活剂（u-PA）、组织型纤溶酶原激活剂（t-PA）和纤溶酶原激活剂抑制剂（PAI）的含量。分别测定各个卵泡液中雌二醇（E2）、孕酮（P）和睾酮（T）的浓度，并将这些酶的水平以及卵泡类固醇含量与各个卵母细胞的受精行为相关联。与含有未受精卵母细胞的卵泡相比，含有可受精卵母细胞的卵泡显示出显著更高的卵泡液E2和P水平以及显著更低的T水平。与未受精的对应颗粒细胞相比，受精卵母细胞的相应颗粒细胞表现出更高水平的t-PA，而u-PA和PAI的水平没有显著差异。这些数据表明，人类卵母细胞的成功受精与颗粒细胞中高含量的t-PA以及卵泡液中高E2和P水平相关。
