Laboratory of Protozoology, Key Laboratory of Ecology and Environment Science in Guangdong Higher Education, College of Life Science, South China Normal University, Guangzhou 510631, PR China.
Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, PR China.
Int J Syst Evol Microbiol. 2015 Jan;65(Pt 1):309-320. doi: 10.1099/ijs.0.067801-0. Epub 2014 Sep 30.
The morphology and morphogenesis of an oxytrichid ciliate, Rubrioxytricha haematoplasma (Blatterer & Foissner, 1990) Berger, 1999, collected from brackish and marine waters in China, were investigated using live observation and the protargol staining method. The main features of the morphogenetic process are: (i) the parental adoral zone of membranelles is retained completely in the proter and the anlage of undulating membranes originates from dedifferentiation of the old structures; (ii) three frontal, four frontoventral, one buccal, five ventral and five transverse cirri are derived from the anlagen of the undulating membranes and the five streaks of frontal-ventral-transverse anlagen in the pattern of 1:3:3:3:4:4 from left to right; (iii) the morphogenesis of the dorsal kineties is simpler than the Oxytricha pattern, i.e. without fragmentation of the dorsal kinety 3 anlagen; (iv) the single caudal cirrus originates from the dorsal kinety 3 anlage on the right side; (v) the two macronuclear nodules fuse into a single mass during the mid-stage of morphogenesis. These features correspond well with Rubrioxytricha indica, indicating that the morphogenetic pattern of Rubrioxytricha is stable. Phylogenetic analysis based on small-subunit rRNA gene sequence data supports the monophyly of the genus Rubrioxytricha, which is nested within the non-Stylonychinae clade.
从中国的咸水和海水环境中收集到的 Rubrioxytricha haematoplasma（Blatterer & Foissner，1990） Berger，1999 的形态和形态发生学，使用活体观察和蛋白银染色方法进行了研究。形态发生过程的主要特征是：（i）亲代口前纤毛带完全保留在原虫体中，波动膜的原基起源于旧结构的去分化；（ii）三个额前、四个额腹、一个口纤毛、五个腹纤毛和五个横纤毛由波动膜的原基和额腹横原基的五条条纹衍生而来，模式为从左到右 1:3:3:3:4:4；（iii）后纤毛带的形态发生比 Oxytricha 模式更简单，即没有后纤毛带 3 的原基碎片化；（iv）单个尾纤毛起源于右侧后纤毛带 3 的原基；（v）两个大核节融合成单个块状物在形态发生的中期。这些特征与 Rubrioxytricha indica 非常吻合，表明 Rubrioxytricha 的形态发生模式是稳定的。基于小亚基 rRNA 基因序列数据的系统发育分析支持 Rubrioxytricha 属的单系性，该属嵌套在非 Styloychinae 分支内。
