Batalla-Carrera Laia, Morton Ana, Shapiro-Ilan David, Strand Michael R, García-Del-Pino Fernando
Departament Biologia Animal, Vegetal i Ecologia. Facultat de Biociències, Universitat Autònoma de Barcelona, Bellaterra, E-08193 Barcelona, Spain.
USDA-ARS, Southeastern Fruit and Tree Nut Research Laboratory, 21 Dunbar Rd., Byron, GA 31008.
J Nematol. 2014 Sep;46(3):281-6.
We investigated the existing susceptibility differences of the hazelnut weevil, Curculio nucum L. (Coleoptera:, Curculionidae) to entomopathogenic nematodes by assessing the main route of entry of the nematodes, Steinernema carpocapsae strain B14 and S. feltiae strain D114, into larvae and adult insects, as well as host immune response. Our results suggested that S. carpocapsae B14 and S. feltiae D114 primarily entered adult insects and larvae through the anus. Larvae were more susceptible to S. feltiae D114 than S. carpocapsae B14 and adults were highly susceptible to S. carpocapsae B14 but displayed low susceptibility to S. feltiae D114. Penetration rate correlated with nematode virulence. We observed little evidence that hazelnut weevils mounted any cellular immune response toward S. carpocapsae B14 or S. feltiae D114. We conclude the differential susceptibility of hazelnut weevil larvae and adults to S. carpocapsae B14 and S. feltiae D114 primarily reflected differences in the ability of these two nematodes to penetrate the host.
我们通过评估线虫斯氏线虫B14菌株和斯氏小卷蛾线虫D114菌株进入榛实象甲幼虫和成虫的主要途径以及宿主免疫反应，研究了榛实象甲（Curculio nucum L.，鞘翅目：象甲科）对昆虫病原线虫的现有易感性差异。我们的结果表明，斯氏线虫B14菌株和斯氏小卷蛾线虫D114菌株主要通过肛门进入成虫和幼虫体内。幼虫对斯氏小卷蛾线虫D114的易感性高于斯氏线虫B14，而成虫对斯氏线虫B14高度易感，但对斯氏小卷蛾线虫D114的易感性较低。穿透率与线虫毒力相关。我们几乎没有观察到榛实象甲对斯氏线虫B14菌株或斯氏小卷蛾线虫D114产生任何细胞免疫反应的证据。我们得出结论，榛实象甲幼虫和成虫对斯氏线虫B14菌株和斯氏小卷蛾线虫D114的易感性差异主要反映了这两种线虫穿透宿主能力的差异。
