Ventura-Mejía Consuelo, Medina-Ceja Laura
Laboratory of Neurophysiology and Neurochemistry, Department of Cellular and Molecular Biology, Centro Universitario de Ciencias Biológicas y Agropecuarias (CUCBA), University of Guadalajara, Camino Ing. R. Padilla Sánchez 2100, Las Agujas, Nextipac, Zapopan, JAL, CP 45110, Mexico.
Biomed Res Int. 2014;2014:282490. doi: 10.1155/2014/282490. Epub 2014 Sep 3.
In models of temporal lobe epilepsy and in patients with this pathology, high frequency oscillations called fast ripples (FRs, 250-600 Hz) can be observed. FRs are considered potential biomarkers for epilepsy and, in the light of many in vitro and in silico studies, we thought that electrical synapses mediated by gap junctions might possibly modulate FRs in vivo.
Animals with spontaneous recurrent seizures induced by pilocarpine administration were implanted with movable microelectrodes in the right anterior and posterior hippocampus to evaluate the effects of gap junction blockers administered in the entorhinal cortex. The effects of carbenoxolone (50 nmoles) and quinine (35 pmoles) on the mean number of spontaneous FR events (occurrence of FRs), as well as on the mean number of oscillation cycles per FR event and their frequency, were assessed using a specific algorithm to analyze FRs in intracranial EEG recordings.
We found that these gap junction blockers decreased the mean number of FRs and the mean number of oscillation cycles per FR event in the hippocampus, both during and at different times after carbenoxolone and quinine administration.
These data suggest that FRs may be modulated by gap junctions, although additional experiments in vivo will be necessary to determine the precise role of gap junctions in this pathological activity associated with epileptogenesis.
在颞叶癫痫模型及患有该病症的患者中，可观察到一种名为快速涟漪（FRs，250 - 600赫兹）的高频振荡。FRs被认为是癫痫的潜在生物标志物，并且鉴于许多体外和计算机模拟研究，我们认为由缝隙连接介导的电突触可能在体内调节FRs。
对通过毛果芸香碱诱导产生自发性复发性癫痫发作的动物，在右侧前海马体和后海马体植入可移动微电极，以评估在内嗅皮质中给予缝隙连接阻滞剂的效果。使用一种特定算法分析颅内脑电图记录中的FRs，评估羧苄青霉素（50纳摩尔）和奎宁（35皮摩尔）对自发性FR事件的平均数量（FRs的发生情况）、每个FR事件的振荡周期平均数量及其频率的影响。
我们发现，在给予羧苄青霉素和奎宁期间及之后的不同时间，这些缝隙连接阻滞剂均降低了海马体中FRs的平均数量以及每个FR事件的振荡周期平均数量。
这些数据表明FRs可能受缝隙连接调节，尽管还需要进行更多的体内实验来确定缝隙连接在这种与癫痫发生相关的病理活动中的精确作用。
