Taschwer Magdalena, Weiß Jennifer A, Kunert Olaf, Schmid Martin G
Department of Pharmaceutical Chemistry, Institute of Pharmaceutical Sciences, Karl-Franzens-University Graz, Universitätsplatz 1, A-8010 Graz, Austria.
Department of Pharmaceutical Chemistry, Institute of Pharmaceutical Sciences, Karl-Franzens-University Graz, Universitätsplatz 1, A-8010 Graz, Austria.
Forensic Sci Int. 2014 Nov;244:e56-9. doi: 10.1016/j.forsciint.2014.09.007. Epub 2014 Sep 22.
Novel psychoactive drugs, such as amphetamine-, cathinone-, benzofury- and tryptamine derivatives, gained high popularity on the global drug market in the last years. These drugs are sold via the Internet as for example "research chemicals", "room odorizers" or "lawn fertilizers" by different online suppliers. They are also known as "Legal Highs", among them, cathinone derivatives play an important role. Well known substituted cathinone derivatives are mephedrone, brephedrone and flephedrone. Since a couple of weeks, a chlorine substituted methcathinone derivative, namely clephedrone (4-chloromethcathinone), is commercially available via the Internet from www.deboralabs.com. The goal of this study was to confirm identity of this substance, which was done successfully by GC-MS and NMR. Since all cathinone derivatives are chiral, it was found out, whether the purchased sample was present as a racemic mixture. For this purpose, methods for enantioseparation by GC and CE were developed and applied successfully. In case of CE a chiral selector was added, whereas chiral separation with GC-MS was done indirectly, after derivatization of clephedrone with trifluoroacetyl-l-prolyl chloride.
新型精神活性物质，如苯丙胺、卡西酮、苯并呋喃和色胺衍生物，在过去几年中在全球毒品市场上广受欢迎。这些毒品通过互联网由不同的在线供应商作为“研究化学品”“房间除臭剂”或“草坪肥料”出售。它们也被称为“合法兴奋剂”，其中卡西酮衍生物起着重要作用。知名的取代卡西酮衍生物有甲麻黄碱、溴麻黄碱和氟麻黄碱。几周以来，一种氯取代的甲基卡西酮衍生物，即氯麻黄碱（4-氯甲基卡西酮），可通过互联网从www.deboralabs.com上购得。本研究的目的是确认该物质的身份，通过气相色谱-质谱联用仪（GC-MS）和核磁共振（NMR）成功完成了这一目标。由于所有卡西酮衍生物都是手性的，因此要确定所购买的样品是否以消旋混合物的形式存在。为此，开发并成功应用了气相色谱法（GC）和毛细管电泳法（CE）进行对映体拆分。在毛细管电泳法中添加了一种手性选择剂，而在气相色谱-质谱联用仪中，氯麻黄碱用三氟乙酰-L-脯氨酰氯衍生化后间接进行手性分离。
