Doğan Ayşegül, Demirci Selami, Bayir Yasin, Halici Zekai, Karakus Emre, Aydin Ali, Cadirci Elif, Albayrak Abdulmecit, Demirci Elif, Karaman Adem, Ayan Arif Kursat, Gundogdu Cemal, Sahin Fikrettin
Department of Genetics and Bioengineering, Faculty of Engineering and Architecture, Yeditepe University 34755 Istanbul, Turkey.
Department of Biochemistry, Faculty of Pharmacy, Ataturk University, 25240, Erzurum, Turkey.
Mater Sci Eng C Mater Biol Appl. 2014 Nov;44:246-53. doi: 10.1016/j.msec.2014.08.035. Epub 2014 Aug 17.
Scaffold-based bone defect reconstructions still face many challenges due to their inadequate osteoinductive and osteoconductive properties. Various biocompatible and biodegradable scaffolds, combined with proper cell type and biochemical signal molecules, have attracted significant interest in hard tissue engineering approaches. In the present study, we have evaluated the effects of boron incorporation into poly-(lactide-co-glycolide-acid) (PLGA) scaffolds, with or without rat adipose-derived stem cells (rADSCs), on bone healing in vitro and in vivo. The results revealed that boron containing scaffolds increased in vitro proliferation, attachment and calcium mineralization of rADSCs. In addition, boron containing scaffold application resulted in increased bone regeneration by enhancing osteocalcin, VEGF and collagen type I protein levels in a femur defect model. Bone mineralization density (BMD) and computed tomography (CT) analysis proved that boron incorporated scaffold administration increased the healing rate of bone defects. Transplanting stem cells into boron containing scaffolds was found to further improve bone-related outcomes compared to control groups. Additional studies are highly warranted for the investigation of the mechanical properties of these scaffolds in order to address their potential use in clinics. The study proposes that boron serves as a promising innovative approach in manufacturing scaffold systems for functional bone tissue engineering.
基于支架的骨缺损修复由于其骨诱导和骨传导性能不足，仍然面临许多挑战。各种生物相容性和可生物降解的支架，结合适当的细胞类型和生化信号分子，在硬组织工程方法中引起了极大的兴趣。在本研究中，我们评估了将硼掺入聚（丙交酯-乙交酯-酸）（PLGA）支架中，无论有无大鼠脂肪来源干细胞（rADSCs），对体内外骨愈合的影响。结果显示，含硼支架增加了rADSCs的体外增殖、附着和钙矿化。此外，在股骨缺损模型中，应用含硼支架通过提高骨钙素、血管内皮生长因子（VEGF）和I型胶原蛋白水平，促进了骨再生。骨矿化密度（BMD）和计算机断层扫描（CT）分析证明，施用含硼支架提高了骨缺损的愈合率。与对照组相比，将干细胞移植到含硼支架中可进一步改善与骨相关的结果。为了研究这些支架在临床上的潜在用途，非常有必要对其力学性能进行进一步研究。该研究表明，硼是制造功能性骨组织工程支架系统的一种有前景的创新方法。
