Ye Chuan, He Zhixu, Lin Yunfeng, Zhang Yi, Tang Jin, Sun Bo, Ma Minxian, Liu Jielin, Yang Long, Ren Houxiang, Zhao Baoping
Department of Orthopaedics, The Affiliated Hospital of Guiyang Medical College, Guiyang, China,
Biotechnol Lett. 2015 Feb;37(2):449-56. doi: 10.1007/s10529-014-1693-0. Epub 2014 Oct 4.
Bio-electrospraying (BES) is a technique for directly jetting living cells that has significant implications for tissue engineering and regenerative medicine. However, the effect of BES on human adipose-derived stem cells (hASCs) remains unknown. Here, we show that an hASC suspension was successfully electrosprayed via a continuous, stable and linearly directed electrospray at 10 kV and at 3 ml/h. Morphological observations and Trypan Blue and CCK-8 assays revealed that the cells remained viable and proliferated at a rate similar to that of the controls (0 kV). However, at 20 kV, BES became unstable and cell viability was reduced. Moreover, hASCs electrosprayed at 10 kV retained their multilineage potential, successfully differentiating into chondrogenic, osteogenic and neurogenic lineages. Thus, BES does not significantly affect cell morphology, viability or multipotency.
生物电喷雾（BES）是一种直接喷射活细胞的技术，对组织工程和再生医学具有重要意义。然而，BES对人脂肪来源干细胞（hASC）的影响尚不清楚。在此，我们表明，hASC悬浮液在10 kV电压和3 ml/h流速下通过连续、稳定且呈线性定向的电喷雾成功实现了电喷雾。形态学观察以及台盼蓝和CCK-8检测显示，细胞保持存活且增殖速率与对照组（0 kV）相似。然而，在20 kV时，BES变得不稳定且细胞活力降低。此外，在10 kV下进行电喷雾的hASC保留了其多向分化潜能，成功分化为软骨、成骨和神经谱系。因此，BES不会显著影响细胞形态、活力或多能性。
