富含桑椹花色苷果汁的肽组学研究。

Peptidomics study of anthocyanin-rich juice of elderberry.

机构信息

Department of Chemistry, Hong Kong Baptist University, Kowloon Tong, Hong Kong SAR, PR of China; Department of Chemistry, University of Missouri, Columbia, MO, USA; Center for Botanical Interaction Studies, University of Missouri, Columbia, MO, USA.

Department of Chemistry, University of Missouri, Columbia, MO, USA; Center for Botanical Interaction Studies, University of Missouri, Columbia, MO, USA.

出版信息

Talanta. 2015 Jan;131:640-4. doi: 10.1016/j.talanta.2014.08.022. Epub 2014 Aug 27.

DOI:10.1016/j.talanta.2014.08.022

PMID:25281152

Abstract

Biologically active peptides play a role in plant signaling and defense. Elderberry juice is known to contain a variety of anthocyanin compounds, a sub-set of polyphenols, which are responsible for the deep purple color of the juice. In this paper, we describe a method utilizing solid phase extraction (SPE) to remove anthocyanins from peptides. Liquid chromatography coupled with tandem mass spectrometry was used to separate and identify the peptides. The results showed that the use of SPE was an effective method to separate peptides from anthocyanins and other background compounds including high polyphenol content in the juice samples. More than 1000 peptides present in elderberry juice were successfully identified.

摘要

生物活性肽在植物信号转导和防御中发挥作用。已知接骨木果汁中含有多种花色苷化合物，这是多酚的一个子集，负责果汁的深紫色。在本文中，我们描述了一种利用固相萃取（SPE）从肽中去除花色苷的方法。液相色谱串联质谱联用用于分离和鉴定肽。结果表明，SPE 是一种有效的方法，可以将肽与花色苷以及其他背景化合物（包括果汁样品中高多酚含量）分离。成功鉴定了接骨木果汁中存在的 1000 多种肽。