Ogata N, Kanai K, Ohkuma H, Uyama M
Nippon Ganka Gakkai Zasshi. 1989 Feb;93(2):187-95.
Previously we reported that retinal pigment epithelial cells (RPE) showed different phagocytotic activity according to the charge characteristics of the surface of polystyrene particles which were injected into the subretinal space of albino rats. In this study we examined whether subretinal mucopolysaccharide controls RPE phagocytosis or not, using positive or negative charged particles (3 microns in diameter). The technique of ruthenium red staining, a cationic dye, was employed with special efforts to detect the relationship between acid mucopolysaccharide and particles at the electron microscopic level. Six hours after injection of the positive charged particles, the ruthenium red staining revealed fine granular electron-dense materials, coating the surfaces of many small substances surrounding polystyrene particles which were not yet phagocytized by RPE. On the other hand, there was no staining of negative ones which were already phagocytized by RPE. At 24 hours, no staining was observed on the surface of either particles. These findings suggest that an anionic property of mucopolysaccharide in the subretinal space controls the phagocytosis of RPE.
此前我们报道过，视网膜色素上皮细胞（RPE）根据注入白化大鼠视网膜下间隙的聚苯乙烯颗粒表面的电荷特性表现出不同的吞噬活性。在本研究中，我们使用带正电或负电的颗粒（直径3微米）来研究视网膜下粘多糖是否控制RPE的吞噬作用。采用钌红染色技术（一种阳离子染料），特别致力于在电子显微镜水平检测酸性粘多糖与颗粒之间的关系。注入带正电颗粒6小时后，钌红染色显示有细小的颗粒状电子致密物质，覆盖在许多尚未被RPE吞噬的聚苯乙烯颗粒周围的小物质表面。另一方面，已经被RPE吞噬的带负电颗粒则没有染色。在24小时时，两种颗粒表面均未观察到染色。这些发现表明，视网膜下间隙中粘多糖的阴离子特性控制着RPE的吞噬作用。
