Doping Control Laboratory (DoCoLab), Department of Clinical Chemistry, Microbiology and Immunology, Ghent University (UGent), Technologiepark 30, B-9052, Zwijnaarde, Belgium.
Drug Test Anal. 2014 Nov-Dec;6(11-12):1155-9. doi: 10.1002/dta.1730. Epub 2014 Oct 6.
In this work, a modified version of the 44 amino acid human growth hormone-releasing hormone (hGHRH(1-44)) containing an N-terminal proline extension, a valine residue in position 14, and a C-terminus amidation (sequence: PYADAIFTNSYRKVVLGQLSARKLLQDIMSRQQGESNQERGARARL-NH2 ) has been identified in a confiscated product by liquid chromatography-high resolution mass spectrometry (LC-HRMS). Investigation of the product suggests also an incomplete C-term amidation. Similarly to other hGHRH analogues, available in black markets, this peptide can potentially be used as performance-enhancing drug due to its growth hormone releasing activity and therefore it should be considered as a prohibited substance in sport. Additionally, the presence of partially amidated molecule reveals the poor pharmaceutical quality of the preparation, an aspect which represents a big concern for public health as well.
在这项工作中，通过液相色谱-高分辨质谱（LC-HRMS）在没收产品中鉴定出一种经过修饰的 44 个氨基酸人生长激素释放激素（hGHRH（1-44）），其包含 N 端脯氨酸延伸、14 位缬氨酸残基和 C 端酰胺化（序列：PYADAIFTNSYRKVVLGQLSARKLLQDIMSRQQGESNQERGARARL-NH2）。对该产品的研究表明，其 C 端酰胺化也不完全。与其他可在黑市上买到的 hGHRH 类似物一样，由于其生长激素释放活性，这种肽可能被用作性能增强药物，因此应将其视为运动中的禁用物质。此外，部分酰胺化分子的存在表明该制剂的药物质量较差，这一方面也对公众健康构成了重大关切。
