棕榈酰辅酶A和花生四烯酰辅酶A合成酶活性在人血小板中的亚细胞分布相同。
Identical subcellular distribution of palmitoyl-CoA and arachidonoyl-CoA synthetase activities in human blood platelets.
作者信息
Bakken A M, Farstad M
机构信息
Laboratory of Clinical Biochemistry, University of Bergen, University Hospital Haukeland, Norway.
出版信息
Biochem J. 1989 Jul 1;261(1):71-6. doi: 10.1042/bj2610071.
Abstract
Fractionation of human blood platelets showed that palmitoyl-CoA synthetase and arachidonoyl-CoA synthetase activities had an identical distribution among subcellular fractions. The activity was highest with arachidonic acid as substrate in all fractions, with an enzyme activity of 50 nmol/min per mg of protein, in a 'dense-tubular-system'-enriched fraction. The ratio activities with arachidonate and palmitate as substrates was about 1.5 in all fractions. Heat inactivation did not distinguish between arachidonoyl-CoA synthetase and a palmitoyl-CoA synthetase. On the other hand, heat inactivation indicated two pools of long-chain acyl-CoA synthetases: one in a mitochondria- and one in the dense-tubular-system-enriched fraction.
摘要
对人血小板进行分级分离显示，棕榈酰辅酶A合成酶和花生四烯酰辅酶A合成酶活性在亚细胞分级组分中的分布相同。以花生四烯酸为底物时，所有分级组分中的活性最高，在富含“致密管状系统”的分级组分中，酶活性为每毫克蛋白质50 nmol/分钟。以花生四烯酸和棕榈酸为底物时，所有分级组分中的活性比约为1.5。热失活无法区分花生四烯酰辅酶A合成酶和棕榈酰辅酶A合成酶。另一方面，热失活表明存在两组长链酰基辅酶A合成酶：一组在线粒体中，另一组在富含致密管状系统的分级组分中。
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本文引用的文献
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Discovery of an arachidonoyl coenzyme A synthetase in human platelets.人血小板中花生四烯酰辅酶A合成酶的发现。
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Isolation and characterization of plasma membranes from krebs II ascite cells using Percoll gradient.使用Percoll梯度从克雷布斯II腹水细胞中分离和鉴定质膜。
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