Zhang D F
Zhonghua Yi Xue Za Zhi. 1989 Mar;69(3):137-9, 12.
Clones of HBsAg-reactive CD8+ and CD4+ T cells were obtained from PBM of a hepatitis B immunized individual whose PBM proliferated when cultured with HBsAg. Lymphocytes were activated by culturing for 2 weeks with HBsAg and high concentrations of IL-2, then cloned in the presence of irradiated HBsAg-activated PBM and autologous EBV-transformed B cells, together with antigen and IL-2. All clones examined exhibited proliferation in an antigen-specific manner. Of 7 clones examined by flow cytometry, 4 were CD4+, CD8-; and 3 were CD4-, CD8+. Several clones produced IL-2 activity after stimulation with HBsAg. Since development of CD8+ T-cell clones specific for soluble antigens has been difficult, the high frequency with which CD8+ cells were cloned in these experiments suggests that the cloning strategy employed may have general use for development of CD8+ clones, Availability of HBV-specific T cell clones of different phenotype may help elucidate the mechanisms of immunotolerance in HB infection.
从一名乙肝免疫个体的外周血单核细胞（PBM）中获得了乙肝表面抗原（HBsAg）反应性CD8⁺和CD4⁺T细胞克隆。该个体的PBM在与HBsAg共培养时会增殖。淋巴细胞通过与HBsAg和高浓度白细胞介素-2（IL-2）培养2周进行激活，然后在经照射的HBsAg激活的PBM和自体EB病毒转化的B细胞存在的情况下，连同抗原和IL-2一起进行克隆。所有检测的克隆均以抗原特异性方式增殖。通过流式细胞术检测的7个克隆中，4个为CD4⁺、CD8⁻；3个为CD4⁻、CD8⁺。几个克隆在受到HBsAg刺激后产生IL-2活性。由于针对可溶性抗原的CD8⁺T细胞克隆的培养一直很困难，在这些实验中CD8⁺细胞的高克隆频率表明所采用的克隆策略可能普遍适用于CD8⁺克隆的培养。不同表型的乙肝病毒特异性T细胞克隆的可得性可能有助于阐明乙肝感染中免疫耐受的机制。
