Lucendo-Villarin Baltasar, Cameron Kate, Szkolnicka Dagmara, Travers Paul, Khan Ferdous, Walton Jeffrey G, Iredale John, Bradley Mark, Hay David C
MRC Centre for Regenerative Medicine, University of Edinburgh.
School of Chemistry, University of Edinburgh.
J Vis Exp. 2014 Sep 26(91):51723. doi: 10.3791/51723.
Currently, one of the major limitations in cell biology is maintaining differentiated cell phenotype. Biological matrices are commonly used for culturing and maintaining primary and pluripotent stem cell derived hepatocytes. While biological matrices are useful, they permit short term culture of hepatocytes, limiting their widespread application. We have attempted to overcome the limitations using a synthetic polymer coating. Polymers represent one of the broadest classes of biomaterials and possess a wide range of mechanical, physical and chemical properties, which can be fine-tuned for purpose. Importantly, such materials can be scaled to quality assured standards and display batch-to-batch consistency. This is essential if cells are to be expanded for high through-put screening in the pharmaceutical testing industry or for cellular based therapy. Polyurethanes (PUs) are one group of materials that have shown promise in cell culture. Our recent progress in optimizing a polyurethane coated surface, for long-term culture of human hepatocytes displaying stable phenotype, is presented and discussed.
目前，细胞生物学的主要局限之一是维持分化细胞的表型。生物基质常用于培养和维持原代及多能干细胞来源的肝细胞。虽然生物基质很有用，但它们只能对肝细胞进行短期培养，限制了其广泛应用。我们试图通过使用合成聚合物涂层来克服这些限制。聚合物是种类最为繁多的生物材料之一，具有广泛的机械、物理和化学性质，可根据需要进行微调。重要的是，此类材料可以按质量保证标准进行规模化生产，并显示出批次间的一致性。如果要在制药测试行业进行高通量筛选或基于细胞的治疗而扩大细胞规模，这一点至关重要。聚氨酯（PU）是一类在细胞培养中显示出前景的材料。本文展示并讨论了我们在优化聚氨酯涂层表面以长期培养具有稳定表型的人肝细胞方面的最新进展。
