Fischer T, Wiegmann K, Pfizenmaier K
Max-Planck-Society, Medical Clinic, University of Göttingen, FRG.
Lymphokine Res. 1989 Fall;8(3):305-9.
We investigated the effects of N-glycosidase treatment on the interferon-gamma (IFN-gamma) induced HLA-DR expression of Colo 205 cells. N-glycosidase treatment resulted in a significant decrease of IFN-gamma induced HLA-DR specific immunofluorescence intensity ranging from complete reduction to approximately 30% of that of untreated control cells depending on the IFN-gamma dose. IFN-gamma binding studies showed that this was due to a severe reduction in IFN-gamma binding capacity of N-glycosidase treated cells. Since the number of cell membrane IFN-gamma receptors (IFN-gamma-Rs) was virtually unchanged as revealed by immunofluorescence analysis with a hIFN-gamma-R specific monoclonal antibody this indicates that N-linked carbohydrates play an important role in signal transduction and ligand binding capacity of the hIFN-gamma-R and strengthens the view that carbohydrate moieties of receptor proteins are of greater functional significance than originally anticipated.
我们研究了N-糖苷酶处理对干扰素-γ（IFN-γ）诱导的Colo 205细胞HLA-DR表达的影响。N-糖苷酶处理导致IFN-γ诱导的HLA-DR特异性免疫荧光强度显著降低，根据IFN-γ剂量的不同，强度从完全降低到未处理对照细胞的约30%不等。IFN-γ结合研究表明，这是由于N-糖苷酶处理的细胞中IFN-γ结合能力严重降低所致。用hIFN-γ-R特异性单克隆抗体进行免疫荧光分析显示，细胞膜IFN-γ受体（IFN-γ-R）的数量实际上没有变化，这表明N-连接的碳水化合物在hIFN-γ-R的信号转导和配体结合能力中起重要作用，并强化了这样一种观点，即受体蛋白的碳水化合物部分具有比最初预期更大的功能意义。
