Mørland H, Mørland B
National Institute of Forensic Toxicology, Norwegian Research Council for Science and the Humanities, Oslo.
APMIS. 1989 Sep;97(9):853-7. doi: 10.1111/j.1699-0463.1989.tb00488.x.
Endocytosis of test particles by human blood monocytes (Mo) was tested in the presence of ethanol (80 mM). Phagocytosis via the Fc (IgG)- or C3b receptors (R) was assessed by an assay in which IgG- or C3b coated sheep erythrocytes (E) were used as test particles. Latex particles were tested in parallel with opsonized E. Phagocytosis of IgG-E was reduced to 67 +/- 5% of control (= without ethanol), while the corresponding value for C3b-E was 164 +/- 26% controls. Phagocytosis of latex particles was not affected by ethanol exposure (91 +/- 8% of control). The receptor functions were also tested without ethanol present during the assays. In this part of the study, Mo were incubated with or without ethanol in autologous serum for 15 min at 37 degrees C. After washing the cells free of ethanol, binding properties of the Fc-R or C3b-R were assessed by a rosette assay. Preincubation with ethanol reduced Fc-R binding, while attachment to C3b-R seemed to be more effective. The experiments thus indicate different effects of ethanol treatment in vitro on phagocytic receptors in human Mo. Control experiments revealed no direct effect of ethanol on the test particles.
在存在乙醇（80 mM）的情况下，测试了人血单核细胞（Mo）对测试颗粒的内吞作用。通过使用IgG或C3b包被的绵羊红细胞（E）作为测试颗粒的试验，评估经由Fc（IgG）或C3b受体（R）的吞噬作用。乳胶颗粒与调理过的E平行进行测试。IgG-E的吞噬作用降低至对照（=无乙醇）的67±5%，而C3b-E的相应值为对照的164±26%。乳胶颗粒的吞噬作用不受乙醇暴露的影响（为对照的91±8%）。在试验过程中也在无乙醇的情况下测试了受体功能。在该研究的这一部分中，将Mo在自体血清中于37℃与乙醇一起或不与乙醇一起孵育15分钟。在将细胞洗涤除去乙醇后，通过玫瑰花结试验评估Fc-R或C3b-R的结合特性。用乙醇预孵育会降低Fc-R结合，而与C3b-R的附着似乎更有效。因此，实验表明体外乙醇处理对人Mo中的吞噬受体有不同影响。对照实验显示乙醇对测试颗粒无直接影响。
