Wooten R S, Ahlquist D A, Anderson R E, Carpenter H A, Pemberton J H, Cortese D A, Ilstrup D M
Division of Gastroenterology, Mayo Clinic, Rochester, Minnesota 55905.
Cancer. 1989 Oct 15;64(8):1569-76. doi: 10.1002/1097-0142(19891015)64:8<1569::aid-cncr2820640802>3.0.co;2-g.
This study documents the affinity of hematoporphyrin derivative (HpD) for human colorectal cancer by analysis of surface fluorescence. Ten patients undergoing elective surgery for primary colorectal cancer received intravenous HpD (2-5 mg/kg) either 3 or 72 hours preoperatively. Fluorescent photographs were taken of freshly resected specimens and analyzed using a computerized videodensitometric technique. This technique subtracted contaminating non-HpD fluorescence and facilitated estimation of HpD tissue levels. The HpD-specific fluorescence localized to tumors in all cases, and the mean HpD concentration was six-fold greater in malignant tissue than in surrounding normal mucosa (0.6 microgram/g versus 0.1 microgram/g, P less than 0.001). The authors conclude that HpD preferentially localizes to human colorectal cancer and this is relevant to the photodetection and phototherapy of colorectal neoplasia.
本研究通过表面荧光分析记录了血卟啉衍生物（HpD）对人结肠直肠癌的亲和力。10例接受原发性结肠直肠癌择期手术的患者在术前3小时或72小时静脉注射HpD（2 - 5mg/kg）。对新鲜切除的标本拍摄荧光照片，并使用计算机视频密度测定技术进行分析。该技术可扣除污染性非HpD荧光，便于估算HpD组织水平。在所有病例中，HpD特异性荧光均定位于肿瘤，恶性组织中的平均HpD浓度比周围正常黏膜高6倍（0.6微克/克对0.1微克/克，P<0.001）。作者得出结论，HpD优先定位于人结肠直肠癌，这与结肠直肠肿瘤的光检测和光疗相关。
