Agrez M V, Wharen R E, Anderson R E, Laws E R, Ilstrup D M, Cortese D A, Shorter R G, Lieber M M
J Surg Oncol. 1983 Nov;24(3):173-6. doi: 10.1002/jso.2930240305.
The selective affinity of hematoporphyrin derivative (HpD) for dimethyl-hydrazine-induced colorectal malignancies in mice was evaluated both visually and by a quantitative microfluorescent photometric technique. A significant correlation was found between higher concentrations of HpD in colon biopsies determined by microfluorescence spectroscopy and the presence of carcinoma cells in these biopsies as identified by histologic examination. Visual inspection of the gross fluorescence of tumor-bearing colons in this experimental model, however, was not helpful in differentiating malignant from nonmalignant tissue. The implications of this study regarding the clinical application of HpD tumor fluorescence for the detection of colorectal carcinoma are discussed.
通过视觉观察和定量显微荧光光度技术，评估了血卟啉衍生物（HpD）对二甲基肼诱导的小鼠结肠恶性肿瘤的选择性亲和力。通过显微荧光光谱法测定的结肠活检组织中较高浓度的HpD与组织学检查确定的这些活检组织中癌细胞的存在之间存在显著相关性。然而，在该实验模型中，通过肉眼观察荷瘤结肠的总体荧光，对于区分恶性组织和非恶性组织并无帮助。讨论了本研究对于HpD肿瘤荧光在结直肠癌检测中的临床应用的意义。
